• Publications
  • Influence
Structural basis for scaffolding-mediated assembly and maturation of a dsDNA virus
  • Dh Chen, M. Baker, +10 authors W. Chiu
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences
  • 10 January 2011
Formation of many dsDNA viruses begins with the assembly of a procapsid, containing scaffolding proteins and a multisubunit portal but lacking DNA, which matures into an infectious virion. ThisExpand
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Accurate model annotation of a near-atomic resolution cryo-EM map
Significance Electron cryomicroscopy is a rapidly growing field for macromolecular structure determination. We establish a computational protocol to construct a de novo atomic model from a cryo-EMExpand
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Visualizing Virus Assembly Intermediates Inside Marine Cyanobacteria
Cyanobacteria are photosynthetic organisms responsible for ∼25% of organic carbon fixation on the Earth. These bacteria began to convert solar energy and carbon dioxide into bioenergy and oxygen moreExpand
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Validated near-atomic resolution structure of bacteriophage epsilon15 derived from cryo-EM and modeling
  • M. Baker, C. Hryc, +8 authors W. Chiu
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences
  • 9 July 2013
High-resolution structures of viruses have made important contributions to modern structural biology. Bacteriophages, the most diverse and abundant organisms on earth, replicate and infect allExpand
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The interdigitated β‐helix domain of the P22 tailspike protein acts as a molecular clamp in trimer stabilization
The P22 tailspike adhesin is an elongated thermostable trimer resistant to protease digestion and to denaturation in sodium dodecyl sulfate. Monomeric, dimeric, and protrimeric folding and assemblyExpand
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Copper and Zinc Ions Specifically Promote Nonamyloid Aggregation of the Highly Stable Human γ-D Crystallin.
Cataract is the leading cause of blindness in the world. It results from aggregation of eye lens proteins into high-molecular-weight complexes, causing light scattering and lens opacity. Copper andExpand
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Resolution and Probabilistic Models of Components in CryoEM Maps of Mature P22 Bacteriophage.
CryoEM continues to produce density maps of larger and more complex assemblies with multiple protein components of mixed symmetries. Resolution is not always uniform throughout a cryoEM map, and itExpand
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Intracellular Assembly of Cyanophage Syn5 Proceeds through a Scaffold-Containing Procapsid † † Supplemental material for this article may be found at http://dx.doi.org/10.1128/JVI.01601-10.
ABSTRACT Syn5 is a marine cyanophage that is propagated on the marine photosynthetic cyanobacterial strain Synechococcus sp. WH8109 under laboratory conditions. Cryoelectron images of thisExpand
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Human CCT4 and CCT5 Chaperonin Subunits Expressed in Escherichia coli Form Biologically Active Homo-oligomers*
Background: The subunit-specific roles of the CCT subunits in the chaperonin, TRiC, have not been elucidated. Results: When expressed in E. coli, CCT4 and CCT5 form TRiC-like homo-oligomeric rings.Expand
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Buried hydrophobic side‐chains essential for the folding of the parallel β‐helix domains of the P22 tailspike
The processive β‐strands and turns of a polypeptide parallel β‐helix represent one of the topologically simplest β‐sheet folds. The three subunits of the tailspike adhesin of phage P22 each containExpand
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