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Overexpression of a transporter gene in a multidrug-resistant human lung cancer cell line.
TLDR
Reversion to drug sensitivity was associated with loss of gene amplification and a marked decrease in mRNA expression, and the mRNA encodes a member of the ATP-binding cassette transmembrane transporter superfamily.
Pharmacological characterization of multidrug resistant MRP-transfected human tumor cells.
TLDR
Transfecting two different eukaryotic expression vectors containing MRP complementary DNA into HeLa cells to study the pharmacological phenotype produced exclusively by overexpression of human MRP indicates that drug-resistant cell lines generated by transfection with MRp complementary DNA display some but not all of the characteristics of MRP-overexpressing cell lines produced by drug selection in vitro.
Overexpression of multidrug resistance-associated protein (MRP) increases resistance to natural product drugs.
TLDR
It is demonstrated that MRP overexpression confers a multidrug resistance phenotype similar to that formerly associated exclusively with elevated levels of P-glycoprotein.
Comparison of the Functional Characteristics of the Nucleotide Binding Domains of Multidrug Resistance Protein 1*
TLDR
This work has used a baculovirus dual expression vector encoding both halves of MRP1 to reconstitute an active transporter and compared the ability of each NBD to be photoaffinity-labeled with 8-azido-[32P]ATP and to trap 8-razido-ADP in the presence of orthovanadate.
Pharmacological characterization of the murine and human orthologs of multidrug-resistance protein in transfected human embryonic kidney cells.
Overexpression of the human multidrug-resistance protein (MRP) causes a form of multidrug resistance similar to that conferred by P-glycoprotein, although the two proteins are only distantly related.
Membrane Topology of the Multidrug Resistance Protein (MRP)
TLDR
N-Glycosylation of Asn19 and Asn23 provides the first direct experimental evidence that MRP has an extracytosolic NH2 terminus, and strongly suggests that the NH2-terminal MSD of MRP contains an odd number of transmembrane helices.
cIRF-3, a new member of the interferon regulatory factor (IRF) family that is rapidly and transiently induced by dsRNA.
TLDR
The cloning of a chicken cDNA is reported, cIRF-3, encoding a protein with a DNA-binding domain similar to that found in the mammalian IRF family of proteins, the first example of a non-mammalian IRF protein.
Reconstitution of ATP-dependent Leukotriene C4 Transport by Co-expression of Both Half-molecules of Human Multidrug Resistance Protein in Insect Cells*
TLDR
It is demonstrated that human MRP produced in insect cells can function as an active transporter of LTC4 and that the NH2- and COOH-proximal halves of the protein can assemble efficiently to form a transporter with functional characteristics similar to those of the intact protein.
Analysis of the intron-exon organization of the human multidrug-resistance protein gene (MRP) and alternative splicing of its mRNA.
TLDR
Analyses of the conservation of intron-exon organization and protein primary structure suggest that the MRP-related transporters evolved from a common ancestor shared with the cystic fibrosis transmembrane conductance regulator, by fusion with one or more genes encoding polytopic membrane proteins.
Cloning and characterization of chicken YB-1: regulation of expression in the liver
TLDR
Several lines of evidence are presented that YB-1 expression in the liver is positively associated with DNA synthesis or cell proliferation, and its binding characteristics indicate that the protein can interact specifically with a number of binding sites for liver-enriched or specific factors.
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