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Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.
A series of recombinant genomes which directed expression of the enzyme chloramphenicol acetyltransferase (CAT) in mammalian cells provided a uniquely convenient system for monitoring the expression of foreign DNAs in tissue culture cells. Expand
Humanization of an anti-p185HER2 antibody for human cancer therapy.
The murine monoclonal antibody mumAb4D5, directed against human epidermal growth factor receptor 2 (p185HER2), specifically inhibits proliferation of human tumor cells overexpressing p185HER2.Expand
High efficiency gene transfer into mammalian cells.
The approach has been to use a transient assay system that allows rapid screening of expression of foreign DNA, which has seen that primate cells, including human cells, can be used in gene transfer experiments at levels sensitive enough to allow detection of single copy gene function. Expand
The Rous sarcoma virus long terminal repeat is a strong promoter when introduced into a variety of eukaryotic cells by DNA-mediated transfection.
The results indicate that the Rous sarcoma virus LTR can direct synthesis of high levels of functional mRNA and has a wide expression range. Expand
High efficiency DNA-mediated transformation of primate cells.
Tissue culture cells from several mammalian species, including three primate lines, were transfected with recombinant vectors carrying Escherichia coli xanthine-guanine phosphoribosyltransferase orExpand
Host-specific activation of transcription by tandem repeats from simian virus 40 and Moloney murine sarcoma virus.
The findings suggest that the tandem repeat elements may interact with host-specific molecules and, furthermore, may constitute one of the elements determining the host range of these eukaryotic viruses. Expand
Intervening sequences increase efficiency of RNA 3' processing and accumulation of cytoplasmic RNA.
Two expression vectors were constructed that differ only in the presence of an intervening sequence (IVS) in their 5'-untranslated leaders, and results imply that splicing is coupled to a polyadenylation/transport pathway. Expand
Expression of recombinant plasmids in mammalian cells is enhanced by sodium butyrate.
The results demonstrate that butyrate affects the early stages of gene activity following DNA uptake at least two levels, and expression of integrated recombinant plasmid genes is reinducible by a second treatment five weeks after initial exposure to this agent. Expand
A generic intron increases gene expression in transgenic mice.
The results suggest that inclusion of the generic intron in cDNA constructs may be valuable in achieving high levels of expression in transgenic mice, and that the hybrid intron is significantly more effective in elevating transgene expression. Expand
Minichromosome assembly of non-integrated plasmid DNA transfected into mammalian cells.
It is demonstrated by a variety of means that mammalian cells are capable of rapidly assembling non-integrated circular plasmids into typical "minichromosomes" containing nucleosomes with a 190 bp repetitive spacing. Expand