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Insect cell culture and applications to research and pest management
TLDR
The history of insect cell culture is covered, some recent advances in the field are recounted, and a vision of the future is offered to reveal how insect cells work is offered.
A cell line derived from the red flour beetle Tribolium castaneum (Coleoptera: Tenebrionidae)
TLDR
This represents the first report of a continuously replicating T. castaneum cell line and it is expected the BCIRL-TcA-CLG1 line will become a useful tool in Tribolium research.
The establishment of a Colorado potato beetle (Coleoptera: Chrysomelidae) pupal cell line
TLDR
The cell line was confirmed to have originated from the Colorado potato beetle by the DAF-PCR technique and did not support replication of Autographa californica nucleopolyhedrovirus (AcMNPV) recombinants carrying the genes for green or blue fluorescent proteins.
Development and partial characterization of heliothine cell lines from embryonic and differentiated tissues
TLDR
The establishment of new cell cultures from differentiated and undifferentiated tissues of budworm and bollworm tissues are described and their initial characterization is described.
In vitro host range studies with a new baculovirus isolate from the diamondback moth Plutella xylostella (L.) (Plutellidae: Lepidoptera)
TLDR
The restriction endonuclease profiles of the three baculoviruses showed similarities but could be readily distinguished from each other, and either HV-AM1 or TN-CL1 would be suitable cell lines for the in vitro production of PxMNPV.
Establishment and characterization of insect cell lines from 10 lepidopteran species
TLDR
Cell lines from selected lepidopteran species were established for the overall purpose of use in baculovirus production, including cell lines from a pyralid, the European corn borer, Ostrinia nubilalis, the diamondback moth,Plutella xylostella, as well as eight noctuids.
Establishment and characterization of an Ostrinia nubilalis cell line, and its response to ecdysone agonists
TLDR
A cell line derived from embryonic tissues of the European corn borer, Ostrinia nubilalis (UMC-OnE), was established in EX-CELL 401 medium containing 10% fetal bovine serum and was responsive to treatments of 20-hydroxyecdysone and the ecdysone agonists.
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