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High level of resistance to proteinase inhibitors may be conferred by proteolytic cleavage in beetle larvae.
Incorporation of genes encoding proteinase inhibitors into oilseed rape genome could confer resistance to Coleoptera, which are the major pests on rape in Europe. A detailed study of the digestiveExpand
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Transgenic Plants for Insect Resistance
Plant genetic engineering offers opportunities for the creation of insect-resistant plants by insertion and expression in planta of entomopathogenic proteins. Two main approaches to obtain suchExpand
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Effects of the cysteine protease inhibitor oryzacystatin (OC-I) on different aphids and reduced performance of Myzus persicae on OC-I expressing transgenic oilseed rape
Abstract When administered in artificial diets, the cysteine protease inhibitor (PI) oryzacystatin I (OC-I) induced moderate but significant growth inhibition on the pea aphid (Acyrthosiphon pisumExpand
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Long‐term effects of soybean protease inhibitors on digestive enzymes, survival and learning abilities of honeybees
We have investigated the effects of long‐term ingestion of two serine proteinase inhibitors (PIs), the Kunitz Soybean trypsin inhibitor (SBTI) and the Bowman‐Birk inhibitor (BBI) on survival,Expand
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Effects of Proteinase Inhibitor Ingestion on Survival, Learning Abilities and Digestive Proteinases of the Honeybee
The impact on beneficial insects of proteinase inhibitors expressed in pest-resistant transgenic crops needs to be assessed before the release of these plants into the environment. Three proteinaseExpand
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Growth stimulation of beetle larvae reared on a transgenic oilseed rape expressing a cysteine proteinase inhibitor.
The resistance of a transgenic line of oilseed rape expressing constitutively the cysteine proteinase inhibitor oryzacystatin I (OCI) was assessed against Psylliodes chrysocephala L. (Coleoptera:Expand
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Two strains of cabbage seed weevil (Coleoptera: Curculionidae) exhibit differential susceptibility to a transgenic oilseed rape expressing oryzacystatin I.
The aim of this study was to assess the potential effect of a transgenic line of oilseed rape expressing oryzacystatin I (OCI) on two strains of cabbage seed weevil. The level of OCI expression inExpand
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A multicomponent, elicitor-inducible cystatin complex in tomato, Solanum lycopersicum.
We assessed the ability of the fungal elicitor arachidonic acid to induce cystatin genes in tomato (Solanum lycopersicum), using a cDNA expression library from arachidonate-treated leaves. The cDNAsExpand
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MsCYS1, a developmentally-regulated cystatin from alfalfa.
Several roles have been attributed to cystatins in plants, ranging from the regulation of host [endogenous] cysteine proteases to the inhibition of herbivorous pest [exogenous] proteases. We reportExpand
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A hybrid, broad-spectrum inhibitor of Colorado potato beetle aspartate and cysteine digestive proteinases.
Protein engineering approaches are currently being devised to improve the inhibitory properties of plant proteinase inhibitors against digestive proteinases of herbivorous insects. Here we engineeredExpand
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