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Iron/ascorbate-induced lipid peroxidation changes membrane fluidity and muscarinic cholinergic receptor binding in rat frontal cortex
Analysis of the effects of the induction of LP in vitro on muscarinic cholinergic (Mch) receptor binding and membrane fluidity in rat brain suggests changes in membrane dynamics as detected by the fluorescent probes may be the cause for alterations in Mch receptor kinetics. Expand
Phosphorodiamidate morpholino antisense oligomers inhibit expression of human cytochrome P450 3A4 and alter selected drug metabolism.
Studies indicate AVI-4557 is an effective and specific inhibitor of CYP3A4 expression and sequence-dependent inhibition of cyclophosphamide-related cytocidal activity in both cell types. Expand
Intracellular delivery strategies for antisense phosphorodiamidate morpholino oligomers.
  • C. Ghosh, P. Iversen
  • Biology, Medicine
  • Antisense & nucleic acid drug development
  • 1 August 2000
Physical methods are optimal for the delivery of neutrally charged PMO into cells in culture, and these methods do not leave residual material that may interfere with the interpretation of targeted gene function. Expand
Identification of the prosthetic group of urocanase. The mode of its reaction with sodium borohydride and of its photochemical reactivation.
Both the reversible reducibility of urocanase and its action spectrum of photoreactivation suggest that uro canase contains an enzyme-bound nicotinamide nucleotide molecule which is essential for enzymic activity. Expand
Evaluation of antisense mechanisms of action.
This chapter discusses the idea that evaluation of the inhibition of gene expression can be defined in quantitative terms consistent with inhibitors of enzymatic reactions and/or receptor antagonists, and the comparison of oligonucleotide chemistries as well as comparisons with alternative methods of inhibition may be described. Expand
Mechanism of promoter activity of the β‐amyloid precursor protein gene in different cell lines: identification of a specific 30 bp fragment in the proximal promoter region
Characterization of the APP regulatory region's interaction with cell type‐specific nuclear factor(s) is important to understand tissue‐specific expression of APP seen in AD subjects. Expand
Leishmania donovani: role of microviscosity of macrophage membrane in the process of parasite attachment and internalization.
Host macrophage infection by the parasite Leishmania donovani is heterogeneous, but it is not clear which factors are responsible for parasite recognition within the macrophages. One possible factorExpand
Efficient DNA transfection in neuronal and astrocytic cell lines
Efficient methods for DNA transfection are important to study gene regulation by devising better ways to deliver DNA into the mammalian cells through electroporation and by the calcium phosphate method. Expand
Interactions between Melatonin, Reactive Oxygen Species, and Nitric Oxide
  • D. Lahiri, C. Ghosh
  • Chemistry, Medicine
  • Annals of the New York Academy of Sciences
  • 1 November 1999
In neuroblastoma cells, the release of NOx as mediated by SNP was significantly inhibited by treatment with carboxy-PTIO, a NO scavenger; SOD-1, superoxide dismutase; and melatonin, and the ROS-scavenging function of melatonin along with its neuroprotective and neurodifferentiating role can be utilized for the prevention of neurodegenerative disorders such as AD. Expand
A Proximal Gene Promoter Region for the β‐Amyloid Precursor Protein Provides a Link between Development, Apoptosis, and Alzheimer's Disease
Because serum starvation contributes to the induction of apoptosis, these results suggest a role of the 30‐bp proximal APP promoter element in enhanced apoptotic neuronal cell death. Expand