• Publications
  • Influence
Psychrophilic enzymes: hot topics in cold adaptation
The ability to thrive at temperatures that are close to, or below, the freezing point of water requires a vast array of adaptations to maintain the metabolic rates and sustained growth compatible with life in these severe environmental conditions.
Psychrophilic microorganisms: challenges for life
The ability of psychrophiles to survive and proliferate at low temperatures implies that they have overcome key barriers inherent to permanently cold environments, and the current knowledge of these adaptations as gained from extensive biochemical and biophysical studies and also from genomics and proteomics is discussed.
Cold-adapted enzymes: from fundamentals to biotechnology.
Psychrophilic enzymes: molecular basis of cold adaptation
All known structural factors and weak interactions involved in protein stability are either reduced in number or modified in order to increase their flexibility, and thermal instability of cold-adapted enzymes is regarded as a consequence of their conformational flexibility.
Psychrophilic enzymes: a thermodynamic challenge.
Some like it cold: biocatalysis at low temperatures.
Enzymes from psychrophilic organisms
The comparison of the 3D structures obtained either by protein modelling or by X-ray crystallography (North Atlantic trypsin) with those of their mesophilic counterparts indicates that the molecular changes tend to increase the flexibility of the structure by a weakening of the intramolecular interactions and by an increase of the interactions with the solvent.
Cold-Adapted β-Galactosidase from the Antarctic Psychrophile Pseudoalteromonas haloplanktis
Assays of lactose hydrolysis in milk demonstrate that P. haloplanktis β-galactosidase can outperform the current commercial β-GalactosIDase from Kluyveromyces marxianusvar and suggest that the cold-adapted β- Galaxia coli could be used to hydrolyze lactose in dairy products processed in refrigerated plants.