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Targeted genome modification of crop plants using a CRISPR-Cas system.
1. Jinek, M. et al. Science 337, 816–821 (2012). 2. Cho, S.W., Kim, S., Kim, J.M. & Kim, J.S. Nat. Biotechnol. 31, 230–232 (2013). 3. Cong, L. et al. Science 339, 819–823 (2013). 4. Mali, P. et al.Expand
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Targeted mutagenesis in Zea mays using TALENs and the CRISPR/Cas system.
Transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems have emerged as powerful tools forExpand
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Efficient and transgene-free genome editing in wheat through transient expression of CRISPR/Cas9 DNA or RNA
Editing plant genomes is technically challenging in hard-to-transform plants and usually involves transgenic intermediates, which causes regulatory concerns. Here we report two simple and efficientExpand
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Genome editing in rice and wheat using the CRISPR/Cas system
Targeted genome editing nucleases, such as zinc-finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs), are powerful tools for understanding gene function and forExpand
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Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes
Substantial efforts are being made to optimize the CRISPR/Cas9 system for precision crop breeding. The avoidance of transgene integration and reduction of off-target mutations are the most importantExpand
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Rapid and efficient gene modification in rice and Brachypodium using TALENs.
In the past few years, the use of sequence-specific nucleases for efficient targeted mutagenesis has provided plant biologists with a powerful new approach for understanding gene function andExpand
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The OsSPL16-GW7 regulatory module determines grain shape and simultaneously improves rice yield and grain quality
The deployment of heterosis in the form of hybrid rice varieties has boosted grain yield, but grain quality improvement still remains a challenge. Here we show that a quantitative trait locus forExpand
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Creation of fragrant rice by targeted knockout of the OsBADH2 gene using TALEN technology.
Fragrant rice is favoured worldwide because of its agreeable scent. The presence of a defective badh2 allele encoding betaine aldehyde dehydrogenase (BADH2) results in the synthesis ofExpand
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Establishing a CRISPR–Cas-like immune system conferring DNA virus resistance in plants
CRISPR–Cas (clustered, regularly interspaced short palindromic repeats–CRISPR-associated proteins) is an adaptive immune system in many archaea and bacteria that cleaves foreign DNA on the basis ofExpand
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Gene replacements and insertions in rice by intron targeting using CRISPR–Cas9
Sequence-specific nucleases have been exploited to create targeted gene knockouts in various plants1, but replacing a fragment and even obtaining gene insertions at specific loci in plant genomesExpand
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