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Distinct kinetic mechanisms of the two classes of Aminoacyl-tRNA synthetases.
TLDR
It is shown that transient burst kinetics provides a distinct mechanistic signature dividing the two classes of tRNA synthetases, and that this distinction has significant downstream effects on protein synthesis. Expand
Genetic Mapping and Exome Sequencing Identify Variants Associated with Five Novel Diseases
TLDR
The results reveal the power of coupling new genotyping technologies to population-specific genetic knowledge and robust clinical data and show evidence to strongly support the pathogenicity of variants identified in TUBGCP6, BRAT1, SNIP1, CRADD, and HARS. Expand
Transfer RNA–Mediated Editing in Threonyl-tRNA Synthetase The Class II Solution to the Double Discrimination Problem
TLDR
The present study suggests that both classes of synthetases use effectively the ability of the CCA end of tRNA to switch between a hairpin and a helical conformation for aminoacylation and editing. Expand
Chemical synthesis of biologically active oligoribonucleotides using beta-cyanoethyl protected ribonucleoside phosphoramidites.
TLDR
It is demonstrated that the combination of standard DNA protecting groups, 5'-O-DMT, N-Bz (Ade and Cyt), N-iBu (Gua), beta-cyanoethyl for phosphate, in conjunction with TBDMS for 2'-hydroxyl protection, constitutes a reliable method for the preparation of fully active RNA. Expand
Zinc ion mediated amino acid discrimination by threonyl-tRNA synthetase
TLDR
This study demonstrates that the zinc ion is neither strictly catalytic nor structural and suggests how the zinc ions ensures that only amino acids that possess a hydroxyl group attached to the β-position are activated. Expand
An aminoacyl-tRNA synthetase paralog with a catalytic role in histidine biosynthesis.
TLDR
A class of aminoacyl-tRNA synthetase-like (HisZ) proteins based on the catalytic core of the contemporary class II histidyl-t RNA Synthetase whose members lack aminoacylation activity but are instead essential components of the first enzyme in histidine biosynthesis ATP phosphoribosyltransferase (HisG). Expand
The first step of aminoacylation at the atomic level in histidyl-tRNA synthetase.
TLDR
The use of both conserved metal ions and arginine in phosphoryl transfer provides evidence of significant early functional divergence of class II aminoacyl-tRNA synthetases. Expand
A Unique Hydrophobic Cluster Near the Active Site Contributes to Differences in Borrelidin Inhibition among Threonyl-tRNA Synthetases*
TLDR
This study illustrates how one class of natural product inhibitors affects aminoacyl-tRNA synthetase function, providing potentially useful information for structure-based inhibitor design. Expand
Transfer RNA and human disease
TLDR
This chapter will review recent literature on the relation of mitochondrial and cytoplasmic tRNA, and enzymes that process tRNAs, to human disease and explore the mechanisms involved in the clinical presentation of these various diseases with an emphasis on neurological disease. Expand
Methods for kinetic and thermodynamic analysis of aminoacyl-tRNA synthetases.
TLDR
Application of these methods to any aaRS system allows the investigator to derive detailed kinetic mechanisms for the activation and aminoacyl transfer reactions, permitting issues of substrate specificity, stereochemical mechanism, and inhibitor interaction to be addressed in a rigorous and quantitative fashion. Expand
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