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Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro
TLDR
The derivation of pluripotent embryonic stem (ES) cells from human blastocysts is described, providing a model to study early human embryology, an investigational tool for discovery of novel growth factors and medicines, and a potential source of cells for use in transplantation therapy. Expand
Human feeders support prolonged undifferentiated growth of human inner cell masses and embryonic stem cells
TLDR
It is shown that human fetal and adult fibroblast feeders support prolonged undifferentiated HES cell growth of existing cell lines and are superior to cell-free matrices supplemented with human or MEF feeder–conditioned medium. Expand
Comparative Evaluation of Various Human Feeders for Prolonged Undifferentiated Growth of Human Embryonic Stem Cells
TLDR
It is reported that two adult skin fibroblast cell lines established in‐house from abdominal skin biopsies supported prolonged undifferentiated hES cell growth for over 30 weekly passages in culture. Expand
Human Wharton’s Jelly Stem Cells Have Unique Transcriptome Profiles Compared to Human Embryonic Stem Cells and Other Mesenchymal Stem Cells
TLDR
The ability to modulate immune responses makes hWJSCs an important compatible stem cell source for transplantation therapy in allogeneic settings without immunorejection and provides a foundation for future functional studies where the exact mechanisms of these unique properties of hW JSCs can be confirmed. Expand
Comparative growth behaviour and characterization of stem cells from human Wharton's jelly.
TLDR
Wharton's jelly stem cells are not as pluripotent as hESC but widely multipotent, and have the advantages of being able to be scaled up easily and not inducing teratomas. Expand
Separation of SSEA-4 and TRA-1–60 Labelled Undifferentiated Human Embryonic Stem Cells from A Heterogeneous Cell Population Using Magnetic-Activated Cell Sorting (MACS) and Fluorescence-Activated
TLDR
Both hESCs and HepG2 cells in the ‘flow-through’ fraction following MACS separation were viable in culture whereas by FACS separation only the HepG 2 cells were viable, indicating that FACS efficiently helps to eliminate the undifferentiated h ESCs based on their cell-surface antigens expressed. Expand
Teratomas from pluripotent stem cells: A clinical hurdle
TLDR
This review addresses the issue of tumorigenesis and discusses approaches by which this concern may be overcome and emphasizes the need to concurrently explore alternative stem cell sources that do not confer the disadvantages of pluripotency but are widely multipotent so as to yield safe desirable tissues for clinical application as soon as possible. Expand
Derivation efficiency, cell proliferation, freeze-thaw survival, stem-cell properties and differentiation of human Wharton's jelly stem cells.
TLDR
It is proposed that human WJSC may be frozen at the same time as UCB in cord blood banks for regenerative medicine purposes. Expand
Comparative Characterization of Cells from the Various Compartments of the Human Umbilical Cord Shows that the Wharton’s Jelly Compartment Provides the Best Source of Clinically Utilizable
TLDR
The results show that when isolating MSCs from the UC, the WJ should be the preferred compartment, and a standardized method of derivation must be used so as to make meaningful comparisons of data between research groups. Expand
Isolation and culture of inner cell mass cells from human blastocysts.
TLDR
This is the first report on the successful isolation of human ICM cells and their continued culture for at least two passages in vitro. Expand
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