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Modification of post-PKS tailoring steps through combinatorial biosynthesis.
TLDR
This review covers the highlights of combinatorial biosynthesis applied on post-polyketide synthase modifying enzymes, such as oxygenases, and covers literature from 1985 to 2002. Expand
The complete gene cluster of the antitumor agent gilvocarcin V and its implication for the biosynthesis of the gilvocarcins.
TLDR
This is the first reported gene cluster encoding the biosynthesis of a benzo[d]naphtho[1,2-b]pyran-6-one aryl C-glycoside antibiotic, which lays the foundation for the detailed studies of its intriguing biosynthetic steps and possibly for the generation of gilvocarcin analogues with improved biological activities through combinatorial biosynthesis. Expand
Oxidative rearrangement processes in the biosynthesis of gilvocarcin V.
TLDR
Various experiments are reported, which include incorporation studies with 18O-labeled precursors and the isolation and structure determination of novel intermediates of gilvocarcin biosynthesis accumulated by mutants, in which two genes encoding monooxygenases responsible for the C-C-bond cleavage of the gil Vocarcin pathway were deleted through targeted PCR. Expand
Deoxysugar Methylation during Biosynthesis of the Antitumor Polyketide Elloramycin by Streptomyces olivaceus
TLDR
It is proposed that methyltransferases ElmMI, ElmMII, and ElmMIII are involved in the biosynthesis of the permethylatedl-rhamnose moiety of elloramycin. Expand
The NDP-sugar co-substrate concentration and the enzyme expression level influence the substrate specificity of glycosyltransferases: cloning and characterization of deoxysugar biosynthetic genes of
TLDR
Analysis of deoxysugar biosynthesis genes of the urdamycin biosynthetic gene cluster revealed a widely common biosynthesis pathway leading to D-olivose and L-rhodinose. Expand
Digitoxosyltetracenomycin C and glucosyltetracenomycin C, two novel elloramycin analogues obtained by exploring the sugar donor substrate specificity of glycosyltransferase ElmGT.
TLDR
The explorations of glycosyltransferase ElmGT from Streptomyces olivaceus Tü 2353, which shows an interesting flexibility regarding its sugar donor substrate, were extended toward various previously unexplored sugar co-substrates, revealing that ElmGT is able to process an activated non-deoxygenated sugar, NDP-D-glucose, as well as NDP-L-digitoxose. Expand
Inactivation of gilGT, Encoding a C‐Glycosyltransferase, and gilOIII, Encoding a P450 Enzyme, Allows the Details of the Late Biosynthetic Pathway to Gilvocarcin V to be Delineated
TLDR
Cross‐feeding experiments in which GE, defuco‐GE, and defucogilvocarcin V (defu co‐GV) were fed to an early blocked mutant of the GV biosynthetic pathway, showed that neither GE nor any of the defUco‐ compounds was an intermediate of the pathway. Expand
Elucidation of the function of two glycosyltransferase genes (lanGT1 and lanGT4) involved in landomycin biosynthesis and generation of new oligosaccharide antibiotics.
TLDR
The high-yielding production of novel oligosaccharide antibiotics using glycosyltransferase gene cassettes generated in a very convenient way proves that glycosYLtransferases can be flexible towards the alcohol substrate. Expand
Deoxysugar Transfer during Chromomycin A3 Biosynthesis in Streptomyces griseus subsp. griseus: New Derivatives with Antitumor Activity
TLDR
A biosynthetic pathway is proposed for the glycosylation events in chromomycin A3 biosynthesis and it is proposed that CmmGII and CmmGI are responsible for the formation of the disaccharide chain by incorporating, in a sequential manner, two d-oliosyl residues to the 8 position of the biosynthetics intermediate prechromomycin C3. Expand