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Proteolytic 18O labeling for comparative proteomics: model studies with two serotypes of adenovirus.
- X. Yao, A. Freas, J. Ramírez, P. Demirev, C. Fenselau
- Chemistry, Medicine
- Analytical chemistry
- 2 May 2001
A new method for proteolytic stable isotope labeling is introduced to provide quantitative and concurrent comparisons between individual proteins from two entire proteome pools or their subfractions.… Expand
A unique Mycobacterium ESX‐1 protein co‐secretes with CFP‐10/ESAT‐6 and is necessary for inhibiting phagosome maturation
The ESX‐1 secretion system plays a critical role in the virulence of Mycobacterium tuberculosis and M. marinum. To date, three proteins are known to be secreted by ESX‐1 and necessary for virulence,… Expand
Characterization of intact microorganisms by MALDI mass spectrometry.
The application of MALDI mass spectrometry to desorb protein biomarkers from intact viruses, bacteria, fungus, and spores is the focus of this review. Instrumentation, sample collection, sample… Expand
Identification of bacteria using mass spectrometry
Myeloid-Derived Suppressor Cells: Immune-Suppressive Cells That Impair Antitumor Immunity and Are Sculpted by Their Environment
Myeloid-derived suppressor cells (MDSC) are a diverse population of immature myeloid cells that have potent immune-suppressive activity. Studies in both mice and humans have demonstrated that MDSC… Expand
Characterization of the protein subset desorbed by MALDI from whole bacterial cells.
This study characterizes various features of the proteins that are detected in MALDI mass spectra when whole bacteria cells are analyzed, in an effort to understand why some proteins are successfully… Expand
Gag proteins of the highly replicative MN strain of human immunodeficiency virus type 1: posttranslational modifications, proteolytic processings, and complete amino acid sequences.
The MN strain of human immunodeficiency virus type 1 was grown in H9 cells, concentrated by centrifugation, and disrupted, and proteins were purified by reversed-phase high-pressure liquid… Expand
Human Proteinpedia enables sharing of human protein data
viewed directly in the MMCD interface or downloaded as a tab-delimited file for viewing with spreadsheet software.We have also built a ‘Miscellanea’ search engine that allows users to filter results… Expand
Kurstakins: a new class of lipopeptides isolated from Bacillus thuringiensis.
- Y. Hathout, Y. P. Ho, V. Ryzhov, P. Demirev, C. Fenselau
- Biology, Medicine
- Journal of natural products
- 15 September 2000
A novel class of lipopeptides was isolated from Bacillus thuringiensis kurstaki HD-1. Four compounds (1-4) were separated by high-performance liquid chromatography and their primary structures… Expand
Identification of Bacillus Spores by Matrix-Assisted Laser Desorption Ionization–Mass Spectrometry
- Y. Hathout, P. Demirev, +6 authors C. Fenselau
- Chemistry, Medicine
- Applied and Environmental Microbiology
- 1 October 1999
ABSTRACT Unique patterns of biomarkers were reproducibly characterized by matrix-assisted laser desorption ionization (MALDI)–mass spectrometry and were used to distinguish Bacillus species members… Expand