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Strategies to facilitate the development of uncloned or cloned infectious full-length viral cDNAs: Apple chlorotic leaf spot virus as a case study
Fast and efficient strategies allowing the rapid generation from TNA extracts of FL-cDNAs under the control of the T7 promoter and the successful inoculation of plants using in vitro transcripts obtained from these uncloned amplification products should have broad applications for the study of "difficult" plant viruses.
Phytovirome Analysis of Wild Plant Populations: Comparison of Double-Stranded RNA and Virion-Associated Nucleic Acid Metagenomic Approaches
A novel bioinformatics strategy was used to assess viral richness not only at the family level but also by determining operational taxonomic units (OTU) following the clustering of conserved viral domains.
Association of Little cherry virus 1 (LChV1) with the Shirofugen stunt disease and characterization of the genome of a divergent LChV1 isolate.
The failure to detect any other viral agent in the V2356 SSD source and the identification of LchV1 in a second, independent, source of the disease suggests that LChV1 isolates could be responsible for the SSD syndrome.
Multiple coat protein mutations abolish recognition of Pepino mosaic potexvirus (PepMV) by the potato rx resistance gene in transgenic tomatoes.
These results demonstrate that the Rx-based sensing system is able to recognize the PepMV CP but, contrary to the situation with PVX, many mutations over a significant stretch of the Pep MV CP allow escape from recognition by Rx.
Characterization of Prunus-infecting apricot latent virus-like Foveaviruses: evolutionary and taxonomic implications.
The complete genomic sequences of four Prunus-infecting Apricot latent virus (ApLV) like isolates were determined and used to analyze the taxonomic position and variability of these viruses. The
Development of a polyvalent RT‐PCR detection assay covering the genetic diversity of Cherry capillovirus A
Comparison of existing detection techniques using a panel of CVA isolates representative of the various phylogenetic groups indicated that dot-blot hybridization assays show high polyvalence but may lack the sensitivity to detect CVA in some samples.
Characterization by Deep Sequencing of Prunus virus T, a Novel Tepovirus Infecting Prunus Species.
The name Prunus virus T (PrVT) is proposed for the new virus, considered as a new member of the genus Tepovirus, even if the level of nucleotide identity with PVT is borderline with the genus demarcation criteria for the family Betaflexiviridae.
Characterization by deep sequencing of divergent plum bark necrosis stem pitting-associated virus (PBNSPaV) isolates and development of a broad-spectrum PBNSPaV detection assay.
Complete sequencing of the divergent isolates allowed the development of a more broad-spectrum detection test targeting a conserved region in the P61 gene and indicate a much broader diversity of PBNSPaV than previously thought.
First Report of Chrysanthemum stunt viroid in Various Cultivars of Argyranthemum frutescens in France.
The results reported here represent the first report of CSVd in Argyranthemum for France and implicate a range of cultivars and may reflect the different physiologies or growing conditions of the two kinds of plants.
Viral Double-Stranded RNAs (dsRNAs) from Plants: Alternative Nucleic Acid Substrates for High-Throughput Sequencing.
This chapter describes an optimized protocol for the extraction of double-stranded RNAs from a wide range of plants and for their random amplification prior to NGS sequencing.