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Imaging Dynamic Redox Changes in Mammalian Cells with Green Fluorescent Protein Indicators*
Changes in the redox equilibrium of cells influence a host of cell functions. Alterations in the redox equilibrium are precipitated by changing either the glutathione/glutathione-disulfide ratioExpand
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Binding and in vitro activities of peptides with high affinity for the nociceptin/orphanin FQ receptor, ORL1.
Fifteen hexapeptides having high affinity for the opioid-like receptor ORL1 were identified from a combinatorial library containing more than 52 million different hexapeptides. The five compoundsExpand
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Generation and use of synthetic peptide combinatorial libraries for basic research and drug discovery
EXISTING methods for the synthesis and screening of large numbers of peptides are limited by their inability to generate and screen the requisite number (millions) of individual peptides1–4 and/orExpand
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Orphanin FQ: receptor binding and analog structure activity relationships in rat brain.
A tritiated form of orphanin FQ (a heptadecapeptide also known as Nociceptin) has been prepared. This radioligand (33 Ci/mmole) was used to develop a radioreceptor assay using rat brain homogenates.Expand
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An all D-amino acid opioid peptide with central analgesic activity from a combinatorial library.
A synthetic combinatorial library containing 52,128,400 D-amino acid hexapeptides was used to identify a ligand for the mu opioid receptor. The peptide, Ac-rfwink-NH2, bears no resemblance to anyExpand
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Mixture-based synthetic combinatorial libraries.
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Selective Ligands for the μ, δ, and κ Opioid Receptors Identified from a Single Mixture Based Tetrapeptide Positional Scanning Combinatorial Library*
A combinatorial library of 6,250,000 tetrapeptides in the mixture based positional scanning format was screened in binding assays for the three opioid receptors, μ, δ, and κ. Three different bindingExpand
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Strategies for the use of mixture-based synthetic combinatorial libraries: scaffold ranking, direct testing in vivo, and enhanced deconvolution by computational methods.
Since its inception more than 20 years ago with highthroughput parallel synthesis for oligonucleotides and peptides, synthetic combinatorial methods have fundamentally advanced the ability toExpand
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The pharmacological properties of a novel MCH1 receptor antagonist isolated from combinatorial libraries.
Melanin-concentrating hormone (MCH) is a neuropeptide that exhibits potent orexigenic activity. In rodents, it exerts its actions by interacting with one receptor, MCH(1) receptor which is expressedExpand
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The use of positional scanning synthetic peptide combinatorial libraries for the rapid determination of opioid receptor ligands.
The application of a new synthetic peptide combinatorial library (SPCL) is described. This library, termed a positional scanning SPCL, contains six positional SPCLs, each of which contains allExpand
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