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The cdk7-cyclin H-MAT1 complex associated with TFIIH is localized in coiled bodies.
Both cyclin H and cdk7 did not colocalize with P80 coilin after disruption of the coiled body, indicating that these proteins are specifically targeted to the small nuclear ribonucleoprotein-containing domain. Expand
Mechanisms and Signals for the Nuclear Import of Proteins
The key mechanisms, pathways, and models underlying the transport of proteins across nuclear pores are analysed and it is shown that nuclear import of proteins is an essential step in regulating gene expression and the replication cycle of several viruses. Expand
Envelope Proteins Derived from Naturally Integrated Hepatitis B Virus DNA Support Assembly and Release of Infectious Hepatitis Delta Virus Particles
It is demonstrated for the first time that at least some of the HBV DNA sequence naturally integrated during infection can produce functional small and large envelope proteins capable of the formation of infectious HDV virions. Expand
Electrophoretic Mobility Shift Assay: Analyzing Protein - Nucleic Acid Interactions
The characterization of protein-nucleic acid interactions is essential not only for understanding the wide range of cellular processes they are involved in, but also the mechanisms underlyingExpand
The topography of chromosomes and genes in the nucleus.
Current ideas on the functional compartmentalization of chromosomes and genes within the nucleus are discussed and it is suggested that the spatial distribution of genes during interphase should play a major role in the subnuclear organization of nonchromatin components. Expand
Hepatitis delta virus ribonucleoproteins shuttle between the nucleus and the cytoplasm.
It is shown that although HDV ribonucleoproteins are mainly detected in the nucleus, they are also present in the cytoplasm of cells infected with HDV or transfected withHDV cDNA, which suggests that HDV RNA mediates export of viral particles to the cy toplasm whereas the delta antigen triggers their reimport into the nucleus. Expand
Proteome analysis of a human liver carcinoma cell line stably expressing hepatitis delta virus ribonucleoproteins.
23 differentially expressed proteins were found to be involved in different cellular pathways and the down regulation of the histone H1-binding protein and of triosephosphate isomerase was confirmed by Real time PCR and the upregulation of the La protein and lamin A/C was validated by western blot. Expand
Transcription-dependent nucleocytoplasmic distribution of hnRNP A1 protein in early mouse embryos.
The results show that the presence of newly synthesised RNAs in the cytoplasm is not sufficient to induce the accumulation of hnRNP A1 in the nucleus, and it is proposed that binding of A1 to nascent transcripts retards its export to the cy toplasm and therefore contributes to its concentration inThe nucleus. Expand
Hepatitis Delta Virus: A Peculiar Virus
The hepatitis delta virus (HDV) is distributed worldwide and related to the most severe form of viral hepatitis, which is the smallest known human virus. Expand
Characterization of the nuclear localization signal of the hepatitis delta virus antigen.
The delta antigen (HDAg) is the only protein encoded by the hepatitis delta virus (HDV) RNA genome and it is found that the HDAg NLS consists of a single stretch of 10 amino acids, EGAPPAKRAR, located in positions 66-75. Expand