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Evaluation of a Real-time PCR-based assay using the lightcycler system for detection of Toxoplasma gondii bradyzoite genes in blood specimens from patients with toxoplasmic retinochoroiditis.
TLDR
A highly sensitive Real-time PCR (Light-cycler, LC-PCR) is developed and evaluated to detect and quantify Toxoplasma gondii B1 and bradyzoite specific genes in serum and peripheral blood mononuclear cells (PBMC) specimens from five immunocompetent subjects with clinically suspected toxoplasmic retinochoroiditis (TRC) or without a suspected T. Gondii infection. Expand
The novel zoonotic COVID-19 pandemic: An expected global health concern.
TLDR
An update on the main features of COVID-19 is provided and possible solutions how to halt the expansion of this novel pandemic are suggested. Expand
Chlamydophila pneumoniae Infection and Its Role in Neurological Disorders
TLDR
The demonstration of C. pneumoniae by histopathological, molecular and culture techniques in the late-onset AD dementia has suggested a relationship between CNS infection with C. tuberculosis and the AD neuropathogenesis, and the role of Chlamydia in the pathogenesis of mental or neurobehavioral disorders including schizophrenia and autism is uncertain and fragmentary and will require further confirmation. Expand
Molecular evidence of Ureaplasma urealyticum and Ureaplasma parvum colonization in preterm infants during respiratory distress syndrome
BackgroundUreaplasma urealyticum and U. parvum have been associated with respiratory diseases in premature newborns, but their role in the pathogenesis of the respiratory distress syndrome (RDS) isExpand
Epstein-Barr virus-specific intrathecal oligoclonal IgG production in relapsing-remitting multiple sclerosis is limited to a subset of patients and is composed of low-affinity antibodies
TLDR
It is suggested that an EBV-specific intrathecal oligoclonal IgG production can occur in a subset of MS patients as part of humoral polyreactivity driven by chronic brain inflammation. Expand
Efficacy of a novel reverse transcriptase-polymerase chain reaction (RT-PCR) for detecting Toxoplasma gondii bradyzoite gene expression in human clinical specimens.
TLDR
Findings suggest that RT-PCR method is able to identify the bradyzoite stage of T. gondii especially in patients who are at risk for TE relapse, and has been shown to be more sensitive and specific than n- PCR. Expand
A Case of Leprosy in Italy: A Multifaceted Disease Which Continues to Challenge Medical Doctors
TLDR
Due to the increasing rate of the migration flows, in Italy and in Europe, leprosy should be considered among the differential diagnosis in patients with cutaneous and neurological signs, especially when originating from endemic countries. Expand
Clinical and diagnostic management of toxoplasmosis in the immunocompromised patient.
TLDR
Clinical manifestations, diagnostic procedures and management strategies for toxoplasmosis can be improved by performing real-time PCR protocols and classical serological diagnosis is often inconclusive. Expand
Leishmaniasis in Sardinia. I. Observations on a larval breeding site of Phlebotomus perniciosus, Phlebotomus perfiliewi perfiliewi and Sergentomyia minuta (Diptera: Psychodidae) in the canine
TLDR
This is the first finding of an abundant larval breeding site of P. perniciosus close to the village of Soleminis, a canine leishmaniasis focus 15 km from Cagliari, Sardinia. Expand
Activity of terbinafine against Pneumocystis carinii in vitro and its efficacy in the treatment of experimental pneumonia.
TLDR
Preliminary results suggest further explorations of the terbinafine in clinical trials for treatment and prophylaxis of P. carinii pneumonia. Expand
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