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Complete genome characterization of Sugarcane yellow leaf virus from India: Evidence for RNA recombination
TLDR
The high incidence of SCYLV recombination suggests that recombination plays an important role inSCYLV evolution. Expand
Genetic variability and potential recombination events in the HC-Pro gene of sugarcane streak mosaic virus
TLDR
Analysis of selection pressure indicated that the HC-Pro gene of SCSMV is under strongnegative selection, and it is likely that recombination, along with strong negative selection, enhances the speed of elimination of deleterious mutations in the HC -Pro gene. Expand
Genetic Diversity of Sugarcane Grassy Shoot (SCGS)-Phytoplasmas Causing Grassy Shoot Disease in India
TLDR
Results proved that the phytoplasma isolates infecting sugarcane in India with phenotypic variations from different regions have similar ITS region and further studies are needed to locate other genes in phy toplasma genome which determine variations in phenotypesic expression in the SCGS disease. Expand
Impact of Sugarcane yellow leaf virus (ScYLV) infection on physiological efficiency and growth parameters of sugarcane under tropical climatic conditions in India
TLDR
The present study is the first comprehensive report demonstrating that YL disease caused by ScYLV seriously affects cane and juice yield in major sugarcane varieties under tropical climatic conditions (India), and this situation warrants a massive programme to provide healthy seed material and initiate breeding for YL resistance in Sugarcane. Expand
Diagnosis of Sugarcane yellow leaf virus in asymptomatic sugarcane by RT-PCR
TLDR
Standardized RT-PCR technique with a new set of primers to detect SCYLV in sugarcane in pre-symptomatic stages of YLD clearly revealed that most of the varieties have detectable level of the virus in asymptomatic stage. Expand
Quantification of sugarcane yellow leaf virus in sugarcane following transmission through aphid vector, Melanaphis sacchari
TLDR
The relative standard curve method in RT-qPCR efficiently detected the increment in SCYLV copy numbers in sugarcane following transmission through M. sacchari, showing that the aphid species M.sacchari acts as an effective vector ofSCYLV. Expand
Molecular characterization of Indian sugarcane streak mosaic virus isolate
TLDR
Phylogenetic tree analysis of the complete genome sequences of SCSMV isolates revealed that they can be clustered into two groups and showed 18 % divergence within the highly conserved 3′ partial genome, suggesting a high level of genetic diversity among the Indian SCSMv isolates. Expand
Development of Duplex-Immunocapture (Duplex-IC) RT-PCR for the Detection of Sugarcane streak mosaic virus and Sugarcane mosaic virus in Sugarcane
TLDR
This is the first report on the detection of SCSMV and SCMV together in an IC-RT-PCR assay, and it is concluded that although DAC-ELISA is simple and cost effective to diagnose these viruses, in samples with very low virus titre, IC- RT- PCR would be more sensitive. Expand
Molecular characterization of Indian Sugarcane streak mosaic virus isolates reveals recombination and negative selection in the P1 gene.
TLDR
It is likely that recombination identified in Indian SCSMV isolates, along with strong purifying selection, enhances the speed of elimination of deleterious mutations in the P1 gene. Expand
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