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Porin activity of the native and recombinant outer membrane protein Oms28 of Borrelia burgdorferi.
The outer membrane-spanning (Oms) proteins of Borrelia burgdorferi have been visualized by freeze-fracture analysis but, until recently, not further characterized. We developed a method for theExpand
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Molecular cloning and sequence analysis of the gene encoding OmpL1, a transmembrane outer membrane protein of pathogenic Leptospira spp.
Pathogenic Leptospira spp. are spirochetes that have a low transmembrane outer membrane protein content relative to that of enteric gram-negative bacteria. In a previous study we identified a 31-kDaExpand
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Temporal Analysis of the Antigenic Composition of Borrelia burgdorferi during Infection in Rabbit Skin
ABSTRACT The numbers of host-adapted Borrelia burgdorferi (HAB) organisms in rabbit skin were assessed by real-time PCR over the first 3 weeks of infection. Maximal numbers were found at day 11,Expand
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A Vault Nanoparticle Vaccine Induces Protective Mucosal Immunity
Background Generation of robust cell-mediated immune responses at mucosal surfaces while reducing overall inflammation is a primary goal for vaccination. Here we report the use of a recombinantExpand
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Virulent strain associated outer membrane proteins of Borrelia burgdorferi.
We have isolated and purified outer membrane vesicles (OMV) from Borrelia burgdorferi strain B31 based on methods developed for isolation of Treponema pallidum OMV. Purified OMV exhibited distinctExpand
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Cloning, sequencing, and expression of two class B endoflagellar genes of Treponema pallidum subsp. pallidum encoding the 34.5- and 31.0-kilodalton proteins.
Two structural endoflagellar genes of Treponema pallidum that encode the 34.5- and 31.0-kilodalton (kDa) polypeptides as detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis wereExpand
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A 9.0-kilobase-pair circular plasmid of Borrelia burgdorferi encodes an exported protein: evidence for expression only during infection.
In this study, we report the cloning, sequencing, and molecular analysis of a gene located on a 9.0-kbp circular plasmid of virulent Borrelia burgdorferi B31 designated eppA (exported plasmid proteinExpand
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Isolation of the outer membranes from Treponema pallidum and Treponema vincentii.
The outer membranes from Treponema pallidum subsp. pallidum and Treponema vincentii were isolated by a novel method. Purified outer membranes from T. pallidum and T. vincentii following sucroseExpand
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Complement activation limits the rate of in vitro treponemicidal activity and correlates with antibody-mediated aggregation of Treponema pallidum rare outer membrane protein.
A modification of the in vitro immobilization assay together with freeze-fracture analysis was used to determine the factors responsible for the prolonged time required in vitro to achieve killing ofExpand
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Antigenic Composition of Borrelia burgdorferi during Infection of SCID Mice
ABSTRACT The general concept that during infection of mice the Borrelia burgdorferi surface protein composition differs profoundly from that of tick-borne or in vitro-cultivated spirochetes is wellExpand
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