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Identification of the cohesion molecule, contact sites B, of Dictyostelium discoideum.
Polyspecific antibodies were raised against vegetative cells of Dictyostelium discoideum, strain Ax2, and it was shown that CMC 1 Fab' bound to both the cytoplasm and the surface of fixed cells and also bound to thesurface of live cells. Expand
Dual role for Dictyostelium contact site B in phagocytosis and developmental size regulation
Using a specific antibody against gpl26, it is shown that contact site B is a phagocytosis receptor and contact site A is involved in regulating the size of aggregates formed during morphogenesis; it also seems to be involved in cell–substratum adhesion but is not a developmental trigger. Expand
Hormones, receptors, and cellular interactions in plants
The biochemistry and physiology of cyclic AMP in higher plants Russell P. Newton and Eric G. Brown explore the role of receptors in host-pathogen interactions and possible functions of plant lectins. Expand
Coh A, a mutation affecting the post aggregative related (par) cohesive system of Dictyostelium discoideum
Genetic evidence presented here indicates that the two mutations have been separated by parasexual recombination yielding a Coh A, Rde A+ segregant class of which strain JC-36 is a prototype. Expand
Glucosylation of phosphorylpolyisoprenol and sterol at the plasma membrane of soya-bean (Glycine max) protoplasts.
Although the synthesis of dolichol phosphate glucose and sterol glucosides occurred throughout the sucrose gradient, the specific activities of both glucosyltransferases were greatest in a fraction coincident with the radioactively labelled plasma membrane. Expand
Changes in the Cell Surface Level of GP126 during Development of Dictyostelium discoideum
- C. Chadwick
- 1 April 1986
The developmental regulation of the vegetative cohesion molecule, gp126, has been monitored in the cellular slime mould Dictyostelium discoideum and by radio‐iodination of vegetative cells followed by liquid‐scintillation counting of gp 126, developmental‐regulation could be determined quantitatively. Expand
Stage-specific cohesion of cell ghosts and plasma-membrane fragments from Dictyostelium discoideum.
- C. Chadwick, P. Collodi, M. Sussman
- Biology, Medicine
- Differentiation; research in biological diversity
- 1 July 1985
Ghosts prepared from cells of the wild-type and of a cohesion-defective mutant that were harvested during growth and at aggregation and postaggregative stages of fruiting-body construction exhibited the same cohesive properties as the cells from which they were derived. Expand
Aggregation-related and post-aggregation-related cohesive systems in Dictyostelium discoideum involve stage-specific, Ca2+-dependent, di- or polymeric associations of membrane-bound moieties.
It is shown that the ghosts of newly aggregation-competent cells and of cells from the 'Mexican hat (18 h) stage' of fruit construction can bind, each to its immobilized counterpart, but not heterologously, and suggests that both cohesive acts involve at least dimeric associations of molecules or molecular complexes located within or on juxtaposed membranes. Expand
A path analytic investigation of interrole conflict and organizational commitment related to performance ranking
- C. Chadwick
- 1 December 1996