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First isolation of Flexibacter maritimus from farmed Senegalese sole (Solea senegalensis, Kaup) in Spain
The present study deals with the first isolation of Flexibacter maritimus from Senegalese sole, Solea senegalensis (Kaup), cultured in Spain. After a 24 hours shipment from one fish farm to another,Expand
Improved growth of Flavobacterium psychrophilum using a new culture medium
Abstract The comparative study of different media used for cultivation of Flavobacterium psychrophilum showed that tryptone–yeast extract–salts supplemented with glucose, named FLP medium, was theExpand
Antigenic differences among aquareoviruses correlate with previously established genogroups.
ABSTFUCT: Five aquareoviruses, selected to include 1 from each of the 5 genogroups established by Lupiani et al. (1993; Virology 19?:4?5-479), were compared by cross-neutralization and immunodot testExpand
Replication and morphogenesis of the turbot aquareovirus (TRV) in cell culture
Abstract The ultrastructural aspects of the replication cycle of the turbot aquareovirus (TRV) were studied using CHSE-214 cells. Although this virus in general followed the typical pattern ofExpand
Detection of Flexibacter maritimus in fish tissue using nested PCR amplification.
A nested polymerase chain reaction (PCR) system was developed for the detection of Flexibacter maritimus from fish tissue. The total procedure for the diagnosis of marine flexibacteriosis, from theExpand
Rapid and low-level toxic PCR-based method for routine identification of Flavobacterium psychrophilum.
  • C. Cepeda, Y. Santos
  • Biology, Medicine
  • International microbiology : the official journal…
  • 1 December 2000
We describe a rapid, low-toxicity and simple method for the detection of the bacterial fish pathogen Flavobacterium psychrophilum. The method, based on the polymerase chain reaction (PCR), combinedExpand
Marine environment as reservoir of birnaviruses from poikilothermic animals
Abstract In this study 51 aquatic birnaviruses were isolated from environmental samples (molluscs, wild fish and sediments) during routine surveys around fish farms located in Galicia (NW of Spain).Expand
Efficacy of Chemical Disinfectants against Turbot Aquareovirus.
The susceptibility of turbot aquareovirus to five chemical agents was examined. Treatment with 5 mg of malachite green per liter or 500 mg of iodine per liter resulted in a 90% reduction in virusExpand
Occurrence of an erythrocytic virus infection in cultured turbot Scophthalmus maximus
Cytoplasmic inclusions, ranging in slze from 0.3 to 1 pm, were found in circulating erythrocytes of diseased turbot Scophthalmus maxirnus ( L . ) , reared on several farms In Galicia ( N W Spaln).Expand
Effect of the turbot aquareovirus on fish macrophages using an in vitro model
The turbot aquareovirus TRV has an invasive behaviour, as the vlrus spreads throughout most flsh organs and tissues In the present study the presence of TRV Inside macrophages of both naturally andExpand