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Selection of Suitable Reference Genes for RT-qPCR Analyses in Cyanobacteria
TLDR
This is the first work on the validation of reference genes for cyanobacteria constituting a valuable starting point for future works on RT-qPCR data normalization.
Methyloversatilis universalis gen. nov., sp. nov., a novel taxon within the Betaproteobacteria represented by three methylotrophic isolates.
TLDR
Based on physiological, phenotypic and genomic characteristics of the three isolates, a new genus, Methyloversatilis gen. nov., is proposed within the family Rhodocyclaceae, and the type strain is FAM5T, which represents a novel taxon.
Infection levels and diversity of anisakid nematodes in blackspot seabream, Pagellus bogaraveo, from Portuguese waters
TLDR
The anisakid nematode communities were relatively similar in mainland waters but very distinct in both the Azores and Madeira islands, suggesting the existence of at least three different stocks of P. bogaraveo in the northeast Atlantic.
Improving a Synechocystis-based photoautotrophic chassis through systematic genome mapping and validation of neutral sites
TLDR
The genome mapping and validation of five neutral sites in the chromosome of Synechocystis sp.
Expanding the toolbox for Synechocystis sp. PCC 6803: validation of replicative vectors and characterization of a novel set of promoters
TLDR
It is demonstrated that the presence of the plasmid does not lead to an evident phenotype or hindered Synechocystis growth, being the vast majority of the cells able to retain the replicative plasmids even in the absence of selective pressure.
Novel pollutant-resistant methylotrophic bacteria for use in bioremediation.
TLDR
Thirty one novel methylotrophic bacterial strains were isolated from a range of soil and sediment sources under different enrichment regimes and can be considered real 'super-bugs' for their ability to withstand extremely high concentrations of a variety of pollutants.
Identification of inner membrane translocase components of TolC‐mediated secretion in the cyanobacterium Synechocystis sp. PCC 6803
TLDR
It is suggested that the hyper-vesiculating phenotype of the TolC-deficient mutant is related to cell envelope stress management, highlighting how both classical (TolC) and nonclassical (OMVs-mediated) secretion systems are crucial for cyanobacterial cell homeostasis.
Labrys portucalensis sp. nov., a fluorobenzene-degrading bacterium isolated from an industrially contaminated sediment in northern Portugal.
A detailed classification of a novel bacterial strain, designated F11(T), capable of degrading fluorobenzene as a sole carbon and energy source, was performed by using a polyphasic approach. This
Long-term performance and microbial dynamics of an up-flow fixed bed reactor established for the biodegradation of fluorobenzene
An up-flow fixed bed reactor (UFBR) was established to investigate the biodegradation of fluorobenzene (FB) under a number of operating conditions, which included variation in the concentration of FB
Isolation and properties of a pure bacterial strain capable of fluorobenzene degradation as sole carbon and energy source.
TLDR
Strain F11 belongs to a novel genus within the alpha-2 subgroup of the Proteobacteria, possibly within a new clade related to the order Rhizobiales, and was shown to be able to use a range of other organic compounds, including other fluorinated compounds.
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