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Influenza B virus neuraminidase can synthesize its own inhibitor.
BACKGROUND Neuraminidase, one of the two surface glycoproteins of influenza virus, cleaves terminal sialic acid residues from glycolipids or glycoproteins. Its crystal structure is known at highExpand
Isolation, Purification, and Characterization of an Arabinogalactan from a Red Wine
A polysaccharide originating from grape was purified from a young red wine by affinity, gel permeation, and ion exchange chromatography. Methylation structural analysis and enzymatic degradation byExpand
In vivo fucosylation of lacto-N-neotetraose and lacto-N-neohexaose by heterologous expression of Helicobacter pylori α-1,3 fucosyltransferase in engineered Escherichia coli
We report here the in vivo production of type 2 fucosylated-N-acetyllactosamine oligosaccharides in Escherichia coli. Lacto-N-neofucopentaose Galβ1-4GlcNAcβ1-3Galβ1-4(Fucα1-3)Glc,Expand
Structure of an extracellular polysaccharide produced by Lactobacillus rhamnosus strain C83.
The extracellular polysaccharide produced by Lactobacillus rhamnosus strain C83 was found to be composed of D-glucose and D-galactose in a molar ratio of 2:3. The primary structure of theExpand
Large scale in vivo synthesis of globotriose and globotetraose by high cell density culture of metabolically engineered Escherichia coli.
Large amounts of globotriose (Galalpha-4Galbeta-4Glc) are shown to be produced by the high cell density culture of an Escherichia coli strain over-expressing the Neisseria meningitidis lgtC gene forExpand
Gas-liquid chromatography and mass spectrometry of oligosaccharides obtained by partial acetolysis of glycans of glycoproteins.
Abstract A method is described for the analysis and identification of oligosaccharides obtained by acetolysis of glycoproteins. Oligosaccharides were analyzed by gas-liquid chromatography massExpand
Production of Lewis x Tetrasaccharides by Metabolically Engineered Escherichia coli
Two tetrasaccharides carrying the trisaccharidic Lewis x motif on a GlcNAc or a Gal residue were produced on the gram‐scale by high‐cell‐density cultures of metabolically engineered Escherichia coliExpand
The living factory: In vivo Production of N-acetyllactosamine containing carbohydrates in E. coli
Scientific and commercial interest in oligosaccharides is increasing, but their availability is limited as production relies on chemical or chemo-enzymatic synthesis. In search for a more economical,Expand