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Dephosphorylation by calcineurin regulates translocation of Drp1 to mitochondria
TLDR
A role for calcineurin-dependent translocation of the profission dynamin related protein 1 (Drp1) to mitochondria in dysfunction-induced fragmentation is shown and it is shown that fragmentation of depolarized mitochondria depends on a loop involving sustained Ca2+ rise, activation of calcineURin, and dephosphorylation of Drp1 and its translocation to the organelle.
Hereditary spastic paraplegia proteins REEP1, spastin, and atlastin-1 coordinate microtubule interactions with the tubular ER network.
TLDR
Data indicate that the HSP proteins atlastin-1, spastin, and REEP1 interact within the tubularER membrane in corticospinal neurons to coordinate ER shaping and microtubule dynamics.
Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)
TLDR
There continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes, so it is important to update guidelines for monitoring autophagic activity in different organisms.
Cyclic AMP-dependent Protein Kinase Phosphorylation of Drp1 Regulates Its GTPase Activity and Mitochondrial Morphology*
TLDR
Protein phosphorylation at Ser637 results in clear alterations in Drp1 function and mitochondrial morphology that are likely involved in dynamic regulation of mitochondrial division in cells.
Cellular pathways of hereditary spastic paraplegia.
  • C. Blackstone
  • Biology, Medicine
    Annual review of neuroscience
  • 20 June 2012
TLDR
A range of studies are converging on alterations in the shaping of organelles, particularly the endoplasmic reticulum, as well as intracellular membrane trafficking and distribution as primary defects underlying the HSPs, with clear relevance for other long axonopathies affecting peripheral nerves and lower motor neurons.
Atlastin GTPases are required for Golgi apparatus and ER morphogenesis.
TLDR
The atlastin family of GTPases functions prominently in both ER and Golgi morphogenesis, but they do not appear to be required generally for anterograde ER-to-Golgi trafficking.
Release of OPA1 during Apoptosis Participates in the Rapid and Complete Release of Cytochrome c and Subsequent Mitochondrial Fragmentation*
TLDR
It is demonstrated that cytochrome c release during apoptosis precedes mitochondrial fragmentation, and it is suggested that an initial mitochondrial leak of OPA1 leads to cristae structural alterations and exposure of previously sequestered protein pools, permitting continued release in a feed-forward manner to completion.
Structural basis for midbody targeting of spastin by the ESCRT-III protein CHMP1B
TLDR
The endosomal sorting complex required for transport (ESCRT) machinery, including ESCRT-III, localizes to the midbody and participates in the membrane-abscission step of cytokinesis and reveals a specific, high-affinity complex involving a noncanonical binding site between the first and third helices of the MIT domain.
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