Pseudomonas syringae Phytotoxins: Mode of Action, Regulation, and Biosynthesis by Peptide and Polyketide Synthetases
- C. Bender, F. Alarcón-Chaidez, D. Gross
- Biology, ChemistryMicrobiology and Molecular Biology Reviews
- 1 June 1999
Genetic reprogramming of peptide and polyketide synthetases has been successful, and portions of the coronatine and syringomycin gene clusters could be valuable resources in developing new antimicrobial agents.
The complete genome sequence of the Arabidopsis and tomato pathogen Pseudomonas syringae pv. tomato DC3000
- C. Buell, V. Joardar, A. Collmer
- Biology, EngineeringProceedings of the National Academy of Sciences…
- 19 August 2003
The complete genome sequence of the model bacterial pathogen Pseudomonas syringae pathovar tomato DC3000 (DC3000), which is pathogenic on tomato and Arabidopsis thaliana, is reported and 1,159 genes unique to DC3000 are revealed, of which 811 lack a known function.
Virulence systems of Pseudomonas syringae pv. tomato promote bacterial speck disease in tomato by targeting the jasmonate signaling pathway.
- You-Xing Zhao, R. Thilmony, C. Bender, A. Schaller, S. He, G. Howe
- BiologyThe Plant Journal
- 1 November 2003
Results indicate that COR promotes bacterial virulence by activating the host's JA signaling pathway, and further suggest that the type III secretion system might also modify host defense by targeting theJA signaling pathway in susceptible tomato plants.
Identification and characterization of a well-defined series of coronatine biosynthetic mutants of Pseudomonas syringae pv. tomato DC3000.
- D. M. Brooks, G. Hernández-Guzmán, B. Kunkel
- BiologyMolecular Plant-Microbe Interactions
- 1 February 2004
This is the first study to use biochemically and genetically defined COR mutants to address the role of COR in pathogenesis and constructed and characterized cfa6 cmaA double mutant strains, suggesting that reduced virulence of these mutants on A. thaliana is caused by the absence of the intact COR toxin.
The phytotoxin coronatine and methyl jasmonate impact multiple phytohormone pathways in tomato.
The results indicate that conjugation of CFA to an amino acid is required for optimal activity in tomato, including chlorosis, changes in chloroplast structure, cell wall thickening, accumulation of proteinase inhibitors, induction of anthocyanins, and root growth inhibition.
Pseudomonas syringae manipulates systemic plant defenses against pathogens and herbivores.
- J. Cui, Adam K. Bahrami, F. Ausubel
- Environmental ScienceProceedings of the National Academy of Sciences…
- 1 February 2005
It is reported that virulent strains of the bacterial phytopathogen Pseudomonas syringae induce systemic susceptibility to secondary P. syringsae infection in the host plant Arabidopsis thaliana, and data suggest that SIS may be a consequence of the previously described mutually antagonistic interaction between the salicylic acid and JA signaling pathways.
The Pseudomonas syringae phytotoxin coronatine promotes virulence by overcoming salicylic acid-dependent defences in Arabidopsis thaliana.
Results indicate that COR is required both for overcoming or suppressing SA-dependent defences during growth in plant tissue and for normal disease symptom development in A. thaliana.
Effects of Environmental and Nutritional Factors on Production of the Polyketide Phytotoxin Coronatine by Pseudomonas syringae pv. Glycinea
The results of the present study were used to formulate a medium which allowed for enhanced coronatine production in nearly all strains of P. syringae tested and a rapid method for extracting coron atine from small volumes of culture supernatant was developed.
The Plant Pathogen Pseudomonas syringae pv. tomato Is Genetically Monomorphic and under Strong Selection to Evade Tomato Immunity
A genome-based micro-evolutionary study of a bacterial plant pathogen, Pseudomonas syringae pv.
Identification and relatedness of coronatine-producing Pseudomonas syringae pathovars by PCR analysis and sequence determination of the amplification products
- S. Bereswill, P. Bugert, B. Völksch, M. Ullrich, C. Bender, K. Geider
- BiologyApplied and Environmental Microbiology
- 1 August 1994
DNA hybridization studies previously indicated that the coronatine biosynthetic gene cluster is highly conserved among P. syringae strains which produce the toxin, and the PCR products obtained from the pathovars maculicola and tomato were the most similar to each other, which supports the hypothesis that these two pathovar are closely related.