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Amplification of a Cytochrome P450 Gene Is Associated with Resistance to Neonicotinoid Insecticides in the Aphid Myzus persicae
The mechanisms underlying resistance to the neonicotinoid insecticides were investigated using biological, biochemical, and genomic approaches and it was suggested that P450-mediated detoxification plays a primary role in resistance, although additional mechanism(s) may also contribute. Expand
The evolution of insecticide resistance in the peach potato aphid, Myzus persicae.
The biochemical and molecular mechanisms underlying resistance in M. persicae are summarized and the insights study of this topic has provided on how resistance evolves, the selectivity of insecticides, and the link between resistance and host plant adaptation are summarized. Expand
Detection of knockdown resistance (kdr) mutations in Anopheles gambiae: a comparison of two new high-throughput assays with existing methods
Two new high-throughput assays were developed and compared with four established techniques for kdr detection, indicating that where maximum sensitivity and specificity are required the TaqMan real-time assay is the preferred method. Expand
Resistance to diamide insecticides in diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae) is associated with a mutation in the membrane-spanning domain of the ryanodine receptor.
A region of the gene encoding the proposed diamide binding site of the RyR from P. xylostella strongly suggests a causal association with diamide resistance, and designed a pyrosequencing-based diagnostic assay for resistance monitoring purposes that can be used to detect the G4946E mutation in field-collected samples of diamondback moth. Expand
The global status of insect resistance to neonicotinoid insecticides.
Despite the current scale of resistance, neonicotinoids remain a major component of many pest control programmes, and resistance management strategies, based on mode of action rotation, are of crucial importance in preventing resistance becoming more widespread. Expand
PCR-based detection of Plasmodium in Anopheles mosquitoes: a comparison of a new high-throughput assay with existing methods
A new high-throughput TaqMan assay is described that very effectively detects the four Plasmodium species that cause malaria in humans and discriminates the most deadly species, P. falciparum, from the others. Expand
Mutation of a nicotinic acetylcholine receptor β subunit is associated with resistance to neonicotinoid insecticides in the aphid Myzus persicae
This study finds a single point mutation in the loop D region of the nAChR β1 subunit of the resistant clone of M. persicae, causing an arginine to threonine substitution (R81T) and provides further validation of exisiting models of neonicotinoid binding and selectivity for insect nA ChRs. Expand
Distribution of insensitive acetylcholinesterase (ace‐1R) in Anopheles gambiae s.l. populations from Burkina Faso (West Africa)
Objective  To investigate through countrywide sampling at 20 localities across the three different agro‐climatic zones of Burkina Faso, the distribution of the acetylcholinesterase insensitiveExpand
Gene amplification and microsatellite polymorphism underlie a recent insect host shift
It is concluded that the mutations leading to overexpression of CYP6CY3 were a prerequisite for the host shift of M. persicae to tobacco and that gene amplification and microsatellite polymorphism are evolutionary drivers in insect host adaptation. Expand
Pyrosequencing the transcriptome of the greenhouse whitefly, Trialeurodes vaporariorum reveals multiple transcripts encoding insecticide targets and detoxifying enzymes
New transcriptomic resources for T. vaporariorum are developed that will serve the community studying this important crop pest and will elucidate further the molecular mechanisms underlying insecticide resistance. Expand