• Publications
  • Influence
Imaging neural activity in worms, flies and mice with improved GCaMP calcium indicators
TLDR
A single-wavelength GCaMP2-based GECI (GCaMP3) is developed, with increased baseline fluorescence, increased dynamic range and higher affinity for calcium, and long-term imaging in the motor cortex of behaving mice revealed large fluorescence changes in imaged neurons over months. Expand
Genes that act downstream of DAF-16 to influence the lifespan of Caenorhabditis elegans
TLDR
The findings suggest that the insulin/IGF-I pathway ultimately exerts its effect on lifespan by upregulating a wide variety of genes, including cellular stress-response, antimicrobial and metabolic genes, and by downregulating specific life-shortening genes. Expand
Optimization of a GCaMP Calcium Indicator for Neural Activity Imaging
TLDR
GCaMP5 fluorescence provides a more reliable measure of neuronal activity than its predecessor GCaMP3, which allows more sensitive detection of neural activity in vivo and may find widespread applications for cellular imaging in general. Expand
Odorant-selective genes and neurons mediate olfaction in C. elegans
TLDR
Through laser ablation, chemosensory neurons that detect volatile odorants are identified and it is shown that the nematode C. elegans might have senses that correspond to smell and taste, respectively. Expand
A circuit for navigation in Caenorhabditis elegans.
TLDR
The roles of individual sensory neurons, interneurons, and motor neurons in exploratory behaviors under different conditions are dissected to suggest this circuit may represent a common substrate for multiple navigation behaviors. Expand
Genetically encoded calcium indicators for multi-color neural activity imaging and combination with optogenetics
TLDR
Red, single-wavelength GECIs, “RCaMPs,” engineered from circular permutation of the thermostable red fluorescent protein mRuby are described and 2-color calcium imaging is demonstrated both within the same cell (registering mitochondrial and somatic [Ca2+]) and between two populations of cells: neurons and astrocytes. Expand
Oxygen sensation and social feeding mediated by a C. elegans guanylate cyclase homologue
TLDR
It is shown that the nematode Caenorhabditis elegans exhibits a strong behavioural preference for 5–12% oxygen, and that social feeding can be a behavioural strategy for responding to hyperoxic environments. Expand
GFP Reconstitution Across Synaptic Partners (GRASP) Defines Cell Contacts and Synapses in Living Nervous Systems
TLDR
GRASP marks known synaptic contacts in C. elegans, correctly identifies changes in mutants with altered synaptic specificity, and can uncover new information about synaptic locations as confirmed by electron microscopy, may prove particularly useful for defining connectivity in complex nervous systems. Expand
Chemosensation in C. elegans.
  • C. Bargmann
  • Biology, Medicine
  • WormBook : the online review of C. elegans…
  • 25 October 2006
TLDR
Chemosensory preferences can be modified by sensory adaptation, developmental history, and associative learning, allowing C. elegans to integrate context and experience into its behavior. Expand
An optimized fluorescent probe for visualizing glutamate neurotransmission
TLDR
An intensity-based glutamate-sensing fluorescent reporter with signal-to-noise ratio and kinetics appropriate for in vivo imaging is described and its utility for visualizing glutamate release by neurons and astrocytes in increasingly intact neurological systems is validated. Expand
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