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Vanadate-resistant yeast mutants are defective in protein glycosylation.
Spontaneous recessive orthovanadate-resistant mutants of Saccharomyces cerevisiae were obtained in five complementation groups, and all show defects in protein glycosylation that mimic the previouslyExpand
Structure and biosynthesis of the mannan component of the yeast cell envelope.
  • C. Ballou
  • Biology, Medicine
  • Advances in microbial physiology
  • 1976
TLDR
The chapter discusses the detailed structures of specific yeast mannans, and investigates the enzymic processes involved in mannan synthesis, using cell-free extracts and following incorporation of radioactive mannose into endogenous and exogenous acceptors. Expand
Studies on the enzyme enolase. I. Equilibrium studies.
  • F. Wold, C. Ballou
  • Biology, Medicine
  • The Journal of biological chemistry
  • 1 July 1957
Method for Fingerprinting Yeast Cell Wall Mannans
Controlled acetolysis of yeast mannans yields mixtures of oligosaccharides with (1→2) and (1→3) linkages between the mannose units, whereas the less stable (1→6) linkages of the polysaccharideExpand
Revision of the oligosaccharide structures of yeast carboxypeptidase Y.
The N-linked oligosaccharides from baker's yeast carboxypeptidase Y were analyzed by 1H NMR and specific mannosidase digestion and found to be identical to those from the Saccharomyces cerevisiaeExpand
Studies on the enzyme enolase. II. Kinetic studies.
  • F. Wold, C. Ballou
  • Biology, Medicine
  • The Journal of biological chemistry
  • 1 July 1957
Characterization of the carbohydrate fragments obtained from Saccharomyces cerevisiae mannan by alkaline degradation.
TLDR
Methylation analysis demonstrated that fragments of mannose-containing polysaccharide of the mannan are attached to glucosamine, and it is probable that the linkage to protein is through a di-N-acetylchitobiose to asparagine as in many other glycoproteins. Expand
A study of the immunochemistry of three yeast mannans.
  • C. Ballou
  • Medicine, Chemistry
  • The Journal of biological chemistry
  • 10 March 1970
TLDR
Rabbit antisera have been prepared against three yeasts whose mannans have closely related but unique structures, and the immunochemical bases for these cross-reactions were determined by a study of the inhibition of the precipitin reactions with oligosaccharides obtained from the mannan by acetolysis. Expand
Cell-cell recognition in yeast: purification of Hansenula wingei 21-cell sexual agglutination factor and comparison of the factors from three genera.
TLDR
Haploid cells of the yeasts Pichia amethionina and Saccharomyces kluyveri also show a constitutive sexual agglutination, and little or no crossreactivity is observed in heterologous mixtures. Expand
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