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Expression of hepatitis B surface antigen in transgenic plants.
Tobacco plants were genetically transformed with the gene encoding hepatitis B surface antigen (HBsAg) linked to a nominally constitutive promoter. Enzyme-linked immunoassays using a monoclonalExpand
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Oral immunization with a recombinant bacterial antigen produced in transgenic plants
The binding subunit of Escherichia coli heat-labile enterotoxin (LT-B) is a highly active oral immunogen. Transgenic tobacco and potato plants were made with the use of genes encoding LT-B or an LT-BExpand
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Edible vaccine protects mice against Escherichia coli heat-labile enterotoxin (LT): potatoes expressing a synthetic LT-B gene.
The authors have designed and constructed a plant-optimize synthetic gene encoding the Escherichia coli heat-labile enterotoxin B subunit (LT-B), for use in transgenic plants as an edible vaccineExpand
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Expression of Norwalk virus capsid protein in transgenic tobacco and potato and its oral immunogenicity in mice.
Alternatives to cell culture systems for production of recombinant proteins could make very safe vaccines at a lower cost. We have used genetically engineered plants for expression of candidateExpand
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Chloroplast protein phosphorylation couples plastoquinone redox state to distribution of excitation energy between photosystems
In photosynthetic membranes isolated from pea leaves, the redox state of the plastoquinone pool controls both the level of phosphorylation of the chloroplast light-harvesting pigment–protein complexExpand
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Production of hepatitis B surface antigen in transgenic plants for oral immunization
Here we present data showing oral immunogenicity of recombinant hepatitis B surface antigen (HBsAg) in preclinical animal trials. Mice fed transgenic HBsAg potato tubers showed a primary immuneExpand
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Human immune responses to a novel norwalk virus vaccine delivered in transgenic potatoes.
A new approach for delivering vaccine antigens is the use of inexpensive, plentiful, plant-based oral vaccines. Norwalk virus capsid protein (NVCP), assembled into virus-like particles, was used as aExpand
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Immunogenicity in humans of a recombinant bacterial antigen delivered in a transgenic potato
Compared with vaccine delivery by injection, oral vaccines offer the hope of more convenient immunization strategies and a more practical means of implementing universal vaccination programsExpand
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High‐level rapid production of full‐size monoclonal antibodies in plants by a single‐vector DNA replicon system
Plant viral vectors have great potential in rapid production of important pharmaceutical proteins. However, high‐yield production of hetero‐oligomeric proteins that require the expression andExpand
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Oral immunization with hepatitis B surface antigen expressed in transgenic plants
Oral immunogenicity of recombinant hepatitis B surface antigen (HBsAg) derived from yeast (purified product) or in transgenic potatoes (uncooked unprocessed sample) was compared. An oral adjuvant,Expand
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