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Most human carcinomas of the exocrine pancreas contain mutant c-K-ras genes
Using in vitro gene amplification by the polymerase chain reaction (PCR) and mutation detection by the RNAase A mismatch cleavage method, we have examined c-K-ras genes in human pancreaticExpand
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Improved Resistance to Controlled Deterioration in Transgenic Seeds1[W][OA]
We show that seed-specific overexpression of the sunflower (Helianthus annuus) HaHSFA9 heat stress transcription factor (HSF) in tobacco (Nicotiana tabacum) enhances the accumulation of heat shockExpand
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Transcriptional activation of a heat shock gene promoter in sunflower embryos: synergism between ABI3 and heat shock factors.
Transient expression analyses in sunflower embryos demonstrated that ABI3, a seed-specific transcription factor from Arabidopsis, activated chimaeric genes with the Ha hsp17.7 G4 promoter. NucleotideExpand
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A method to detect and characterize point mutations in transcribed genes: amplification and overexpression of the mutant c-Ki-ras allele in human tumor cells.
A significant percentage of human tumors contain activated ras oncogenes that have acquired oncogenic potential as a result of somatic point mutations at codon 12 or 61 of the encoded ras geneExpand
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Detection of high incidence of K-ras oncogenes during human colon tumorigenesis
RNAse A mismatch cleavage analysis of 66 primary human colon tumours reveals a high incidence of K-ras genes with mutations at position 12. No apparent correlation was found between the presence ofExpand
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Expression of sunflower low-molecular-weight heat-shock proteins during embryogenesis and persistence after germination: localization and possible functional implications
We isolated and sequenced Ha hsp 17.9, a DNA complementary (cDNA) of dry-seed stored mRNA that encodes a low-molecular-weight heat-shock protein (LMW HSP). Sequence analysis identified Ha hsp17.9,Expand
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A Seed-specific Heat-shock Transcription Factor Involved in Developmental Regulation during Embryogenesis in Sunflower*
We report the cloning and functional characterization of the first heat-shock transcription factor that is specifically expressed during embryogenesis in the absence of environmental stress. InExpand
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Developmental and environmental concurrent expression of sunflower dry-seed-stored low-molecular-weight heat-shock protein and Lea mRNAs
We have cloned and sequenced three different cDNAs from sunflower seed-stored mRNA. Sequence similarities and response to heat-shock identified one of the cDNAs as a low-molecular-weight heat-shockExpand
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A sunflower helianthinin gene upstream sequence ensemble contains an enhancer and sites of nuclear protein interaction.
Genes encoding helianthinin, the major seed protein in sunflower, are highly regulated. We have identified putative cis-acting and trans-acting elements that may function in the control ofExpand
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Hahb‐4, a homeobox‐leucine zipper gene potentially involved in abscisic acid‐dependent responses to water stress in sunflower
Homeodomain-leucine zipper proteins constitute a family of transcription factors found only in plants. We have characterized a full-length cDNA encoding the sunflower homeobox-leucine zipper proteinExpand
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