C. Sprekelsen

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Although transplantation of preserved cartilage has assumed a role of great importance in reconstructive surgery, there are many divergent and contradictory opinions with regard to the outcome of cryopreserved cartilage. This study was formulated to assess the functional state of chondrocytes after cryopreservation. Freeze injury and survival were studied(More)
BACKGROUND The respiratory submucosal glands are a major source of secretions in the airway. Human submucosal laryngeal glands have been scarcely studied, with no works existing about their ultrastructure and histochemistry. METHODS Samples of epiglottis, ventricle, false vocal folds and true vocal folds were fixed in 10% buffered formalin for(More)
An electron-microscopic study of the sensory innervation of human epiglottis was undertaken. The nerve supply of this structure was abundant; numerous free unmyelinated nerve endings of 2.5-3 microns were observed in the stratified epithelium of the epiglottis associated with clear cells containing mitochondria, rough endoplasmic reticulum, microtubules and(More)
Juvenile nasopharyngeal angiofibroma is a source of controversy with respect to therapy because of the many therapeutic modalities that exist, such as classic surgical techniques and newer techniques like nasal endoscopic surgery, which have emerged with the advent of new and better diagnostic techniques. Endoscopic surgery is less aggressive and produces(More)
The presence of immunoreactive nervous fibers in the respiratory nasal mucosa of rats and guinea pigs was studied by means of a modified peroxidase antiperoxidase technique for whole mounting. The fibers with neuropeptide Y (NPY) always appeared in the walls of blood vessels, while the fibers immunoreactive to calcitonin gene-related peptide (CGRP) were(More)
Transplantation of preserved cartilage has an important role in reconstructive surgery. Opinions vary with regard to the performance of cryopreserved cartilage. We studied the functional state of chondrocytes after cryopreservation. Cellular survival was studied using the trypan blue dye exclusion test, a functional assay for cell adhesion, and transmission(More)
The histology of the conchae of the nasal cavity in the guinea pig was investigated by means of conventional light and transmission electron microscopy. The results showed a clear structural difference between the anterior and the posterior portion of the conchae, being in the first zone where the vasculature was more numerous. The arterioles and the(More)
Cartilage grafting is one of the most common procedures in plastic surgery. Since storage of both autologous and allogenic cartilage is necessary, different preservation methods have been used with more or less success. The use of chemical preservation procedures like formaldehyde, Merthiolate or Cialit lead to a loss of the vitality of the graft. This work(More)