C Soler-Claudín

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OBJECTIVE To study the reactions of Mexican HIV-1 positive sera to HIV-2 antigens, and their relation to the mode of transmission and the clinical status of infected individuals. MATERIAL AND METHODS Six-hundred and fifty-four sera samples collected in Mexico were tested using HIV-2 immunoblot (IB) techniques; 492 samples were from HIV-1 positive cases(More)
The use of riboprobes for the detection of RNA viral sequences is analyzed. Subgenomic fragments of cDNA from poliovirus type 2, dengue virus type 4 and human immunodeficiency virus type 1, were inserted downstream SP6 and/or T7 promoters in the transcription vectors pGEM-4z or pSP64. RNAs obtained by in vitro transcription in the presence of UTP infinity(More)
Comprehensive HIV diagnosis of 142 patients was done by ELISA, IgG immunoblot, IgA immunoblot (in patients under 24 months), plasma antigen determination, viral isolation by culture and genome detection by polymerase chain reaction (PCR) amplification. Results showed that 14 patients (10%) with negative or indefinite results for anti-HIV-1 IgG antibody were(More)
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