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1. The kinetic properties of the main conductance state of gamma-aminobutyric acidA (GABA) receptor channels from somata of mouse spinal cord neurones in cell culture were investigated using patch clamp techniques. 2. Whole-cell GABA receptor currents increased in a concentration-dependent manner from 0.5 to 5 microM. 3. Single-channel currents were(More)
1. The effects of the benzodiazepine receptor agonist, diazepam (DZ), and the inverse agonist, methyl-6,7-dimethoxy-4-ethyl-beta-carboline-3-carboxylate (DMCM), on gamma-aminobutyric acid (GABAA) receptor single channel currents were characterized. Outside-out patches were obtained from somata of cultured mouse spinal cord neurones and voltage clamped at(More)
1. The intraburst kinetic properties of the main conductance state of gamma-aminobutyric acidA (GABAA) receptor channels in excised outside-out patches obtained from somata of mouse spinal cord neurones in cell culture were investigated using the patch clamp single-channel recording technique. 2. At 2 microM-GABA, the burst duration distribution was fitted(More)
1. Barbiturate regulation of the kinetic properties of gamma-aminobutyric acidA (GABA) receptor channel chloride currents from somata of mouse spinal cord neurones were investigated using whole-cell and excised outside-out patch-clamp recording techniques. 2. GABA (2 microM), GABA (2 microM) plus phenobarbitone (PhB) (500 microM) and GABA (2 microM) plus(More)
The anticonvulsant activity of diazepam and phenobarbital may be mediated in part by enhancement of inhibition involving gamma-aminobutyric acid (GABA). While both diazepam and phenobarbital increase GABA receptor chloride current, they may have different mechanisms of action, since they bind to different sites on the GABA receptor-chloride channel complex.(More)
Pentobarbital (PB) and picrotoxin (PIC) bind to allosterically coupled sites on the GABAA receptor complex but have opposite effects on GABA receptor currents. PB, an anesthetic/anticonvulsant, enhances, and PIC, a convulsant, inhibits GABA receptor currents. PB and PIC also had opposite effects on single main conductance channel GABA receptor currents(More)
The objective of this paper is to summarize a multi-year effort to advance our understanding in the seismic behavior of, and improve the design of, buildings framed from cold-formed steel (CFS). The effort includes a U.S. National Science Foundation funded project and companion industry-funded projects taken together under the abbreviated name: CFS-NEES.(More)