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SUMMARY Independent component analysis (ICA) has recently been applied to epileptic seizure in the EEG. In this paper, the authors show how the fundamental axioms required for ICA to be valid are broken. Four common cases of childhood seizure are presented and assessed for stationarity and an eigenvalue analysis is applied. In all cases, for the stationary(More)
Previously we developed an active contour method for segmenting and tracking cells in phase-contrast microscopy images. Our method is capable of fine-grained segmentation on noisy image sequences. In this paper, we improve the active contour segmentation model to provide better accuracy, by selectively identifying areas of the contour with low confidence(More)
It is estimated that the adult human brain contains 100 billion neurons with 5-10 times as many astrocytes. Although it has been generally considered that the astrocyte is a simple supportive cell to the neuron, recent research has revealed new functionality of the astrocyte in the form of information transfer to neurons of the brain. In our previous work(More)
BACKGROUND Human neurons (hNT neurons), obtained from the NTera2/D1 precursor cell line, are highly valued by many neuroscientists as isolation of adult human primary neuronal cells continues to elude us. hNT neurons are generated by differentiation of the NT2 precursors for a period of 4 weeks followed by 2 weeks of mitotic inhibition. This yields a(More)
In this communication, we describe a new method which has enabled the first patterning of human neurons (derived from the human teratocarcinoma cell line (hNT)) on parylene-C/silicon dioxide substrates. We reveal the details of the nanofabrication processes, cell differentiation and culturing protocols necessary to successfully pattern hNT neurons which are(More)
In this letter, we use the firing rates from an array of olfactory sensory neurons (OSNs) of the fruit fly, Drosophila melanogaster, to train an artificial neural network (ANN) to distinguish different chemical classes of volatile odorants. Bootstrapping is implemented for the optimized networks, providing an accurate estimate of a network's predicted(More)
We report here the patterning of primary rat neurons and astrocytes from the postnatal hippocampus on ultra-thin parylene-C deposited on a silicon dioxide substrate, following observations of neuronal, astrocytic and nuclear coverage on strips of different lengths, widths and thicknesses. Neuronal and glial growth was characterized 'on', 'adjacent to' and(More)