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Malaria sporozoite infection rates in a mixed species group of 244 Anopheles gambiae Giles sensu lato and 115 An.funestus Giles wild female mosquitoes were compared using three methods to determine cut-off absorbance values for positivity of a Plasmodium falciparum Welch enzyme-linked immunosorbent assay (ELISA). Positive controls were based on P.falciparum(More)
At two sites in the Kisumu area of western Kenya, the species composition of the Anopheles gambiae complex was determined by analysis of ovarian polytene chromosomes. Of 1,915 females, 26.1% were An. arabiensis Patton and 73.9% were An. gambiae Giles; one arabiensis x gambiae hybrid was identified. No major differences in the proportions of An. arabiensis(More)
At 2 sites in western Kenya targeted for future malaria vaccine trials, adult culicine mosquitoes were sampled over one year by 5 collection techniques to assess human exposure to potential vectors of pathogens other than malaria. Collections included 20,910 females representing 19 species in Kisian and 4,312 females of 11 species in Saradidi. Common(More)
This study tested the feasibility of identifying salavary gland sporozoites to species by Plasmodium falciparum ELISA by drying them on slides or in vials. The glands were dissected from Anopheles gambiae Giles s.l. and An. funestus Giles collected in western Kenya. In 119 gland infections containing a geometric mean of 1,222 sporozoites, a mean of 72.5% of(More)
The number of malaria sporozoites in the salivary glands was determined microscopically for 1137 wild, naturally infected Anopheles from western Kenya. Infective Anopheles gambiae Giles sensu lato (n = 874) contained a geometric mean (GM) of 962 sporozoites and An.funestus Giles (n = 263) contained 812. No significant differences were detected in geometric(More)
Blood meals were tested by a direct enzyme-linked immunosorbent assay (ELISA) for 424 Anopheles sergentii and for 63 An. multicolor collected in Siwa, Farafra and Bahariya oases in the Western Desert of Egypt. Both species were highly zoophilic. Human blood-feeding by An. sergentii was less common in Bahariya (2.3%) and Farafra (1.3%) than in Siwa (15.3%).(More)
Direct enzyme-linked immunosorbent assay (ELISA) was used to identify the sources of bloodmeals in phlebotomine sandflies from Baringo District, Rift Valley Province, Kenya. Some bloodmeals had been stored for over 4 years before being analysed. Among 356 sandflies identified, 62.9% were Phlebotomus martini, 14.8% Sergentomyia antennatus, 10% S.schwetzi, 6%(More)
Plasmodium infection rates determined by enzyme-linked immunosorbent assay (ELISA) were compared for Anopheles sergentii (Theobald) and An. multicolor Cambouliu in Siwa Oasis, Egypt, an area with low-level Plasmodium vivax transmission, and in Bahariya and Farafra, two other Egyptian oases which appear to be free of malaria. Initial testing indicated that(More)
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