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In this paper, an alternative procedure for the histochemical detection of HRP using amonium heptamolybdate (AHM) as a stabilizing agent and tetramethyl benzidine (TMB) as a chromogen is reported. This procedure avoids the two main problems that occur in previous methods using sodium nitroferricyanide (SNF) as the stabilizer, namely, the appearance of(More)
The GABAergic innervation of the extraocular motor nuclei in the cat was evaluated using postembedding immunocytochemical techniques. The characterization of GABA-immunoreactive terminals in the oculomotor nucleus was carried out at the light and electron microscopic levels. GABA-immunopositive puncta suggestive of boutons were abundant in semithin sections(More)
Systemic administration of the neurotoxin 3-acetylpyridine to adult lizards results in extensive loss of neurons in the medial cerebral cortex, other brain areas remaining largely unaffected. After the neurotoxic trauma, new cells are produced by mitotic division of cells in the ventricular wall. The new cells migrate along radial glial fibers and replace(More)
Neuronal counts in the medial, dorso-medial, and dorsal areas of the cerebral cortex of a common lizard, Podarcis hispanica, have been performed on a set of different sized (aged) animals. With age, (size), there is a significant increase in the number of neuronal somata of the inner plexiform and granular strata of both the medial and dorso-medial areas.(More)
Calcium-binding proteins have been shown to be excellent markers of specific neuronal populations. We aimed to characterize the expression of calcium-binding proteins in identified populations of the cat extraocular motor nuclei by means of immunohistochemistry against parvalbumin, calretinin, and calbindin D-28k. Abducens, medial rectus, and trochlear(More)
The cerebral cortex of Squamate reptiles (lizards and snakes) may be regarded as an archicortex or "reptilian hippocampus". In lizards, one cortical area, the medial cortex, may be considered as a true "fascia dentata" on grounds of its anatomy, connectivity and cyto- chemo-architectonics of its main zinc-rich axonal projection. Moreover, its late(More)
The morphology, fine structure, and degree of colocalization with GABA, somatostatin, and neuropeptide Y of parvalbumin-containing cells were studied with immunocytochemistry in the cerebral cortex of the lizard Podarcis hispanica. Parvalbumin-containing cells make up a morphologically heterogeneous population of spine-free neurons, displaying the(More)
Intraperitoneal injection of the neurotoxin 3-acetylpyridine (3AP) induces a rapid degeneration of the medial cerebral cortex (lizard fascia dentata) granular layer and of its zinc enriched axonal projection (lizard mossy fibres). After 6-8 weeks post-lesion the cell debris have been removed and the granular layer is repopulated by neurons generated in the(More)
The lizard medial cortex (lizard fascia dentata) is capable of neural regeneration after being lesioned by the anti-metabolite 3-acetylpyridine (3AP). This study was aimed at detecting microglial behaviour during the medial cortex lesion-regeneration process using tomato lectin histochemistry to label microglia (both with light and electron microscopy) and(More)
The number and distribution of GABA- and parvalbumin (PV)-immunoreactive (IR) cells have been studied by immunocytochemistry in the cerebral cortex of newborn and adult lizards. The distribution of GABA-IR cells as well as that of PV-IR cells were similar in newborn and adult lizards, and PV-IR cells were GABA-IR in all cases. However, the absolute number(More)