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After solubilization with zwitterionic detergents 5'-nucleotidase was purified to homogeneity from chicken gizzard. Purified 5'-nucleotidase appeared to be composed of a single polypeptide chain of 79 kDa as revealed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE); gel filtration studies in the presence of detergents, however,(More)
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