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In order to replace the radioactive 51chromium release assay (CRA), a colorimetric method for the determination of cell-mediated cytotoxicity of natural killer (NK) effector cells of dogs and adherent target cells was developed using the dye Rose Bengal (RB). After a 14 h incubation period of leucocytes isolated from the peripheral blood (PBL) of dogs and a(More)
The two mesenchymal cell lines K1 and K6 were established from round cell tumours located at the lips of two dogs. Both cell lines were characterized as being of myelomonocytic origin by morphological, cytochemical, immunocytochemical and functional criteria. In the 51chromium release assay (effector/target cell ratio 100/1) the K6-cell line revealed a mean(More)
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