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Purity testing of recombinant DNA (rDNA) proteins using slab gel electrophoresis in conjunction with scanning densitometry is time consuming and labor intensive and is difficult to reproduce because the dyes used for visualizing proteins do not bind in a stoichiometric fashion for all proteins. The present report describes a micellar capillary zone(More)
The isoelectric points (pIs) of murine monoclonal antibodies (MAbs) determined by published one-step capillary isoelectric focusing (CIEF) methods using simultaneous focusing and electroosmotic mobilization showed appreciable deviation from the pI values determined by the slab gel electrophoretic method. The present report describes a modified one-step CIEF(More)
Protein purity estimation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) coupled with scanning densitometry is a critical component in the manufacture of recombinant proteins for commercial diagnostic assays. However, the procedure is time consuming and often difficult to reproduce because commercial dyes that are used for(More)
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