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The product specified by T4 bacteriophage gene 41 is known from genetic analyses to be essential for phage DNA replication in vivo. Correspondingly, the purified gene 41 protein is an essential component of an efficient in vitro DNA replication system reconstructed from seven purified T4 replication proteins; it is required both for the synthesis of short(More)
The T4 DNA topoisomerase is a recently discovered multisubunit protein that appears to have an essential role in the initiation of T4 bacteriophage DND replication. Treatment of double-stranded circular DNA with large amounts of this topoisomerase in the absence of ATP yields new DNA species which are knotted topological isomers of the double-stranded DNA(More)
In the presence of any natural single-stranded DNA, the bacteriophage T4 gene 41 and gene 61 proteins catalyze the synthesis of a group of pentaribonucleotides. These RNA molecules are homogeneous in chain length, but heterogeneous in sequence. In the presence of the other five T4 replication proteins, these pentaribonucleotides are utilized with high(More)
In the presence of single-stranded DNA, the bacteriophage T4 gene 41 and gene 61 proteins catalyze the synthesis of a group of pentaribonucleotides which are homogeneous in chain length but heterogeneous in nucleotide sequence. When single-stranded T4 DNA is used as template, a unique dinucleoside sequence, pppApC, is found at the 5' end of these(More)
PURPOSE To compare multimodal imaging in detecting lacquer cracks in highly myopic eyes, and to correlate these findings with those of spectral-domain optical coherence tomography (SD-OCT). METHODS An observational case series study. Patients with a refractive error worse than -8 diopters and lacquer cracks were recruited. The rates of detection of the(More)
We have previously reported the development of a multienzyme in vitro DNA replication system which replicates double-stranded (DS) DNA templates efficiently. This system consists of six highly purified bacteriophage T4-encoded proteins, the products of genes 32, 41, 43, 44, 45, and 62 (Morris et al. 1975; Alberts et al. 1975, 1977). Each of these proteins(More)
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