C E Stauffer

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As shown by viscosity and optical rotation dispersion measurements, subtilisin Carlsberg is not denatured in the presence of 10 M urea or 6 M guanidinium chloride. This unusual structural stability made it possible to investigate the effects of these hydrophobic-bond breaking solutes on various aspects of the enzymic interaction with substrates and(More)
We have shown conclusively that during the hydrolysis of N-acylamino acid esters by proteases the substrate bond cleaved is the acyl-carbon-ethereal oxygen bond. This was done by hydrolyzing the ethyl esters in H2180-enriched water, isolating the ethanol formed, and determining the rsO:r60 ratio. The amount of ethanol-l80 found was that of natural(More)