C Büküşoğlu

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We have investigated the rapid steroid effects on intracellular calcium ([Ca2+]i levels in a clonal pituitary cell line (GH3). Among the steroids tested only pregnenolone sulfate induced a rapid and transient [Ca2+]i increase within 1 min. The specificity of pregnenolone sulfate-induced [Ca2+]i increase with respect to steroid structure was pronounced.(More)
BACKGROUND Ischemia-reperfusion injury leads to the activation and endothelial deposition of complement. We investigated whether exposure of human umbilical vein endothelial cells (HUVECs) to hypoxia and/or reoxygenation activates complement and decreases HUVEC-surface expression of the C3 regulatory proteins CD46 and CD55. METHODS AND RESULTS HUVECs were(More)
The radiolabeled progesterone (PG) analogue progesterone-11 alpha-hemisuccinate-(2-[125I]iodohistamine) was used to label PG binding proteins in brain membranes from mouse cerebellum. Photoaffinity labeling and sodium dodecyl sulfate-polyacrylamide gel electrophoresis identified specific PG binding protein bands 1-4 of 64-29 kDa. Bands 1 and 4 were well(More)
Using photoaffinity labeling with the progesterone analogue, progesterone-11 alpha-hemisuccinate-(2-[125I]-iodohistamine) ([125I]-his-PG), we identified and characterized a protein band of MW 29 kDa (p29) in mouse cerebellar membranes whose labeling is markedly inhibited by estrogens. Inhibition of the labeling was specific with respect to steroid(More)
Ethanol is well known to enhance the potencies of a range of anesthetics. However, its effects on steroid anesthesia have not been reported. Because ethanol often is added to the delivery vehicle to increase steroid solubility, it is important to evaluate its contribution to steroid anesthesia. Intravenous injection of ethanol and anesthetic steroid(s)(More)
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