C A van der Velden-de Groot

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Through the developments in molecular biology the interest for large scale animal cell cultivation has sharply increased during the last 5 years. At our laboratory, four different cultivation systems were studied, all of which are homogeneous culture systems, as they lend themselves best for scaling up and for the control of culture conditions. The four(More)
A solid-phase spot enzyme-linked immunosorbent assay (spot-ELISA) using rat monoclonal antibodies (MAbs) and an image-processing system is described. This isotype-specific spot-ELISA permits the enumeration of antibody-secreting cells irrespective of the specificity of the secreted antibodies. When used in combination with an ELISA, the antibody production(More)
To study the effects of the growth rate of the hybridoma cell Mn12 on productivity, cell cycle, cell size, and shear sensitivity, six continuous cultures were run at dilution rate of 0.011, 0.021, 0.023, 0.030, 0.042, and 0.058 h(-1). This particular hybridoma cell appeared to be unstable in continuous culture with respect to specific productivity, as a(More)
Only a decade after Van Wezel introduced the first product made in microcarrier cultures on industrial scale at economically acceptable costs, namely Inactivated Polio Vaccine (IPV), interest was taken in this revolutionary type of cell growth system. The basic idea was to develop a culture system with equal potentials for control of environmental culture(More)
The death rate of Vero cells grown on Cytodex-3 microcarriers was studied as a function of the gas flow rate in a small air-lift loop reactor. The death rate may be described by first-order death-rate kinetics. The first-order death-rate constant as calculated from the decrease in viable cells, the increase in dead cells and the increase in LDH activity is(More)
Serially cultivated kidney cells from captive-bred monkeys appear to be very suitable as cell substrate for the production of inactivated poliovaccine. Up to about the 12th generation level no differences in cell growth and virus yield were observed. Also from a safety point of view, application of such cells is preferable to primary cells from wild-caught(More)
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