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N-Acetyltransferase activity was measured in organ-cultured chick pineal glands. A circadian rhythm of enzyme activity persisted in cultured glands for up to 4 days. The phase of the rhythm in vitro closely approximates its phase in vivo. These observations demonstrate that the pineal gland of chicks contains (or is) a self-sustained circadian oscillator.
The response of the circadian rhythm in N-acetyltransferase (NAT) activity to phase-shifted light cycles was examined in vitro in explant cultures of chick (Gallus domesticus) pineal glands. Bisected portions of glands, obtained from birds housed in a light-dark cycle (LD 12:12), were explanted into culture and maintained under one of three light cycles (LD(More)
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