Céline Davoine

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The interest in reactive electrophile species (RES) stems largely from the fact that they can have powerful biological activities. RES stimulate the expression of cell survival genes as well many other genes commonly upregulated in environmental stress and pathogenesis. RES levels must be carefully controlled in healthy cells but their formation and(More)
Two bursts of H(2)O(2) production have been detected by in situ 3,3'-diaminobenzidine (DAB) staining after cutting of Lolium perenne L. leaf blades. The first burst, which occurred immediately after wounding was inhibited by Na-diethydithiocarbamate (DIECA), a Cu/Zn-superoxide dismutase (SOD) inhibitor. The second burst, which was initiated several hours(More)
Both biotic and abiotic stress activate the oxylipin pathway in plants. As reactive electrophile species (RES), some oxylipins are expected to bind cellular nucleophiles in a Michaël-type addition reaction. Using the HPLC-tandem mass spectrometry techniques, we have established the analytical basis for the investigation of oxylipin conjugation to(More)
Malondialdehyde (MDA) is a small, ubiquitous, and potentially toxic aldehyde that is produced in vivo by lipid oxidation and that is able to affect gene expression. Tocopherol deficiency in the vitamin E2 mutant vte2-1 of Arabidopsis thaliana leads to massive lipid oxidation and MDA accumulation shortly after germination. MDA accumulation correlates with a(More)
Changes in the levels of reactive oxygen species (O2.-, H2O2), and of activities of enzymes involved in their detoxification were investigated during senescence of leaf sheaths of ryegrass stubble. The accumulation of H2O2 in the medium leaf sheaths coincided with a drop in the levels of total glutathione, of pyridine nucleotides and of activities of(More)
Department of Plant Systems Biology, VIB, B–9052 Ghent, Belgium (F.A.H., M.V., S.M., C.S., F.V.B.); Department of Plant Biotechnology and Bioinformatics, Ghent University, B–9052 Ghent, Belgium (F.A.H., M.V., S.M., C.S., F.V.B.); Commissariat à l’Energie Atomique, Direction des Sciences du Vivant, Institut de Biologie Environnementale et Biotechnologie,(More)
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