Cédric Pionneau

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SUPPLEMENTARY METHOD ELISA against CBP-EC peptides Flat-bottom 96-well microtiter plates (Nunc) were coated over night at 4°C with 5 and 25 ng/well peptides (CBP1-EC, CBP1-ECrd, CBP2-EC, CBP2-ECrd) in 50 mM sodium carbonate buffer (pH 9,6). Nonspecific binding sites were blocked for 1 hour at 37°C with PBS containing 2 % non-fat milk. The human sera were(More)
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