Byong H. Lee

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Biochemical characterization of the purified bile salt hydrolase (BSH) from Bifidobacterium bifidum ATCC 11863 revealed some distinct characteristics not observed in other species of Bifidobacterium. The bsh gene was cloned from B. bifidum, and the DNA flanking the bsh gene was sequenced. Comparison of the deduced amino acid sequence of the cloned gene with(More)
The proteolytic system of Bifidobacterium animalis subsp. lactis was analyzed, and an intracellular endopeptidase (PepO) was identified and characterized. This work reports the first complete cloning, purification, and characterization of a proteolytic enzyme in Bifidobacterium spp. Aminopeptidase activities (general aminopeptidases, proline iminopeptidase,(More)
Coenzyme Q10 (CoQ10) is an essential component of the respiratory chain which produces ATP. It is formed from the conjugation of a benzoquinone ring with a hydrophobic isoprenoid chain. Efforts on the production of CoQ10 by microorganisms focus on the development of potent strains by conventional mutagenesis and metabolic engineering especially in(More)
Soybeans and other pulses contain oligosaccharides which may cause intestinal disturbances such as flatulence. This study was undertaken to investigate α -galactosidase-producing probiotics added to frozen foods which can survive warming treatments used in thawing and consumption of the pulses. The maximum α -galactosidase activity (1.26 U/mg protein) was(More)
The inseparable association between man and microbes has long been known and some of their benefits are well documented. However, the use of bacteria as therapeutics has attracted much interest. Much is known about the ability of gut microbes to enhance immunity, lower cholesterol levels, improve gut barrier functions and many more benefits. Although it is(More)
Insecticide efficacy of Beauveria bassiana conidia was improved by optimizing the concentrations of conidial heat-protective saccharides (glucose, sucrose, maltose, trehalose, α-lactose, and mannitol) using response surface methodology. Two field trials in tea gardens were carried out to control leafhopper (Empoasca vitis) by spraying B. bassiana conidia(More)
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