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The morphology and alterations of infective juvenile (J2) body components with emphasis on the body wall, stomatal wall, stylet, and sensilla of Heterodera glycines were observed. During the molt of J2 to J3, the J2 hypodermis separates from the J2 cuticle and forms an extracellular space, continuous with an invagination of the anterior, center of the J3.(More)
Ultrastructural observations of the feeding sites of soybean cyst nematode juveniles 3 days after inoculation of soybean roots revealed the presence of feeding tubes in the host cell syncytium. Feeding tubes, which were extruded from the stylet tips, were formed by products of secretory granules that originated in the dorsal esophageal gland and accumulated(More)
Third-stage infective larvae of Onchocerca volvulus were examined to elucidate the ultrastructure and the interrelations of the stoma, esophagus, intestine, and nervous system. The alimentary canal involves a cuticularized stoma with a triradiate lumen that is continuous with a similar triradiate lumen in the muscular region of the esophagus. The lumen wall(More)
Two different defined growth media were used to culture aseptically the root-knot nematode, Meloidogyne incognita, on excised roots of tomato, Lycopersicon esculentum cv 'Marglobe.' One of these media, STW, was a formulation by Skoog, Tsui, and White and the other, MS, a formulation by Murashige and Skoog. From 1 through 4 weeks, inoculated tissues were(More)
Migration of root-knot larvae (Meloidogyne incognita) into the primary root tissues of red clover (Trifolium pratense, cult. “Kenland”) was accompanied by separation and subsequent compression of cells in front of and along the path of the penetrating nematode. The protoplasts of the parenchymatous cortical cells did not respond to the presence of the(More)
The sites of activity of alkaline phosphatase, acid phosphatase, esterase, peroxidase, adenosine triphosphatase, and cytochrome oxidase were demonstrated histochemically in fresh sections of 'Lee' soybeans infected by the root-knot nematode Meloidogyne incognita acrita. Each of the six enzymes was more active at the sites of infection than in adjacent(More)