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This paper describes the fabrication and use of a biomimetic microfluidic device for the monitoring of a functional porin reconstituted within a miniaturized suspended artificial bilayer lipid membrane (BLM). Such a microfluidic device allows for (1) fluidic and electrical access to both sides of the BLM and (2) reproducible membrane protein insertion and(More)
In this paper, we present a novel microfluidic system dedicated to the application of ultra short pulses (i.e. nanopulses) on cells and the visualization of their effects. Cell plasma membranes can be rendered permeable by the use of nanosecond pulsed electric field. In conventional macroscopic electroporation chambers, the typical pulse duration is on the(More)
This paper deals with the development of a microfluidic biochip for the exposure of living cells to nanosecond pulsed electric fields (nsPEF). When exposed to ultra short electric pulses (typical duration of 3-10ns), disturbances on the plasma membrane and on the intra cellular components occur, modifying the behavioral response of cells exposed to drugs or(More)
This paper proposes to monitor the spheroid’s permeabilization within a dedicated microfluidic device using electrorotation analyses. The combination of two electric solicitations, the negative dielectrophoresis force (nDEP) for the spheroid trapping and the electrorotation torque for its dielectric characterization, is used. An estimation of the spheroid(More)
Human adipose mesenchymal stem cells (haMSCs) are multipotent adult stem cells of great interest in regenerative medicine or oncology. They present spontaneous calcium oscillations related to cell cycle progression or differentiation but the correlation between these events is still unclear. Indeed, it is difficult to mimic haMSCs spontaneous calcium(More)
The use of microfluidic devices for the handling and analysis of suspensions of colloidal gold particles is presented. The plasmonic particles are detected via their resonant light scattering (RLS) in a simple and versatile LED-based dark field illumination geometry. RLS enables both microscopic imaging and microspectroscopy. The measurement of the(More)
This paper investigates the properties of DNA translocations signals in a stochastic framework. The considered signals are relative to the translocation of single strand DNA through natural nanopores, and are obtained using a planar patch clamp method. The stochastic signal analysis is carried out considering the statistical distribution of DNA(More)
A new biochip for adherent cell trapping using micro patterned parylene-C is presented. We demonstrate the ability of micro patterned parylene-C films to provide an efficient positioning and orientation of cells. It is also possible to fix one cell separate from the others which may be crucial in the development of biodevices for the single-cell analysis.(More)