Bruno Le Pioufle

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This paper describes the fabrication and use of a biomimetic microfluidic device for the monitoring of a functional porin reconstituted within a miniaturized suspended artificial bilayer lipid membrane (BLM). Such a microfluidic device allows for (1) fluidic and electrical access to both sides of the BLM and (2) reproducible membrane protein insertion and(More)
Human adipose mesenchymal stem cells (haMSCs) are multipotent adult stem cells of great interest in regenerative medicine or oncology. They present spontaneous calcium oscillations related to cell cycle progression or differentiation but the correlation between these events is still unclear. Indeed, it is difficult to mimic haMSCs spontaneous calcium(More)
A new biochip for adherent cell trapping using micro patterned parylene-C is presented. We demonstrate the ability of micro patterned parylene-C films to provide an efficient positioning and orientation of cells. It is also possible to fix one cell separate from the others which may be crucial in the development of biodevices for the single-cell analysis.(More)
This paper investigates the properties of DNA translocations signals in a stochastic framework. The considered signals are relative to the translocation of single strand DNA through natural nanopores, and are obtained using a planar patch clamp method. The stochastic signal analysis is carried out considering the statistical distribution of DNA(More)
This paper deals with the development of a microfluidic biochip for the exposure of living cells to nanosecond pulsed electric fields (nsPEF). When exposed to ultra short electric pulses (typical duration of 3-10ns), disturbances on the plasma membrane and on the intra cellular components occur, modifying the behavioral response of cells exposed to drugs or(More)
Artificial lipidic bilayers are widely used as a model for the lipid matrix in biological cell membranes. We use the Pockels electro-optical effect to investigate the properties of an artificial lipidic membrane doped with nonlinear molecules in the outer layer. We report here what is believed to be the first electro-optical Pockels signal and image from(More)
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