Bruno F. Marques

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This paper brings a proposal and general specification of a new project that aims to apply Conceptual Blending (Fauconnier and Turner 1998) as a process of creature generation in games. The project we describe is based on Divago (Pereira and Cardoso, 2003 and should be able to bring novelty among creatures that populate a game. Moreover, it is expected to(More)
The deployment of thousands of tiny devices inter-networked together and accessible through the Internet is the result of the increasing trend towards enabling the concepts of Internet-of-Things. As these devices may be scattered in a unplanned way, a routing protocol is needed. The RPL protocol is the IETF proposed standard protocol for IPv6-based(More)
The ability to process high-concentration monoclonal antibody solutions (> 10 g/L) through small-pore membranes typically used for virus removal can improve current antibody purification processes by eliminating the need for feed stream dilution, and by reducing filter area, cycle-time, and costs. In this work, we present the screening of virus filters of(More)
A FRET-based method is used to observe the desorption of di-alkyl peptide nucleic acid amphiphiles (PNAA) from liposomes occurring on binding of complementary DNA oligomers. PNA liposomes were prepared containing fluorescein-labeled PNAA and rhodamine-labeled dipalmitoylphosphoethanolamine (DPPE). These liposomes showed efficient energy transfer from the(More)
We present a method to covalently attach peptide nucleic acid (PNA) to liposomes by conjugation of PNA peptide to charged amino acids and synthetic di-alkyl lipids ("PNA amphiphile," PNAA) followed by co-extrusion with disteroylphosphatidylcholine (DSPC) and cholesterol. Attachment of four Glu residues and two ethylene oxide spacers to the PNAA was required(More)
Using differential scanning calorimetry (DSC), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR), we determined some thermodynamic and structural parameters for a series of amino acid-linked dialkyl lipids containing a glutamic acid-succinate headgroup and di-alkyl chains: C12, C14, C16 and C18 in CHES buffer, pH 10. Upon heating,(More)
Machine-to-Machine technology supports several application scenarios, such as smart metering, automotive, healthcare and city monitoring. Smart metering applications have attracted the interest of companies and governments since these applications bring many benefits (e.g. costs reduction and increased reliability) for production, monitoring and(More)