This article reports on the construction and predictive models for a platform comprised of an engineered tethered membrane. The platform provides a controllable and physiologically relevant environment for the study of the electroporation process. The mixed self-assembled membrane is formed via a rapid solvent exchange technique. The membrane is tethered to… (More)
This paper presents two important engineering aspects of biological ion channels—how to build sensors out of gramicidin channels and how to construct computational models for ion channel permeation. We describe our recent research in these areas, potential challenges and possible solutions.
The nanoscale spacing between a tethered lipid bilayer membrane (tBLM) and its supporting gold electrode can be utilized to determine the polarity selectivity of the conduction of ion channels and ion carriers embedded in a membrane. The technique relies upon a bias voltage sequestering or eliminating ions, of a particular polarity, into or out of the… (More)
This paper studies the construction and predictive models of three novel measurement platforms: (i) a Pore Formation Measurement Platform (PFMP) for detecting the presence of pore forming proteins and peptides, (ii) the Ion Channel Switch (ICS) biosensor for detecting the presence of analyte molecules in a fluid chamber, and (iii) an Electroporation… (More)
Activity of the bacterial mechanosensitive channels of small conductance MscS/MscK of E. coli was investigated under high hydrostatic pressure (HHP) using the "flying-patch" patch-clamp technique. The channels were gated by negative pipette voltage and their open probability was measured at HHP of 0.1 to 80 MPa. The channel open probability decreased with… (More)
This paper deals with the experimental construction, stochastic mod-eling and statistical signal processing of a novel biosensor comprising of biological ion channels. Such nano-scale biosensors are built by incorporating dimeric Gramicidin ion channels into the bilayer membranes of giant unilamellar liposomes and then excising small patches of the membrane… (More)